Honokiol, a Polyphenol Natural Compound, Attenuates Cisplatin-Induced Acute Cytotoxicity in Renal Epithelial Cells Through Cellular Oxidative Stress and Cytoskeleton Modulations

Frontiers in Pharmacology
Tse-En J WangPei-Shiue J Tsai

Abstract

Cisplatin is a potent anti-cancer drug that has been widely used in the treatment of various cancers; however, cisplatin administration results in severe nephrotoxicity and impedes its clinical applications. In this study, we showed that honokiol, a polyphenol constituent extracted from Magnolia officinalis exhibited a short-term protective effect against cisplatin-induced damages in renal epithelial cells in vitro. The protective effects of honokiol were resulted from the combination of (1) reduced cellular oxidative stress ranging from 53 to 32% reduction during a 24-h incubation, (2) the maintenance of cellular antioxidant capacity and (3) the stabilization of cytoskeletal structure of the kidney epithelial cells. By promoting the polymerization of actin (1.6-fold increase) and tubulin (1.8-fold increase) cytoskeleton, honokiol not only maintained epithelial cell morphology, but also stabilized cellular localizations of tight junction protein Occludin and adhesion junction protein E-Cadherin. With stabilized junction protein complexes and structural polymerized cytoskeleton network, honokiol preserved epithelial cell polarity and morphology and thus reduced cisplatin-induced cell disruption and damages. Our data demonstrated...Continue Reading

References

Apr 22, 1994·Journal of Chromatography. B, Biomedical Applications·T H TsaiC F Chen
Jun 2, 1994·The New England Journal of Medicine·E M Fish, B A Molitoris
May 30, 1996·The New England Journal of Medicine·R ThadhaniJ V Bonventre
Feb 12, 1998·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·M K KuhlmannH Köhler
Jan 29, 1999·Physiological Reviews·C YeamanW J Nelson
Jun 7, 2002·Critical Reviews in Oncology/hematology·Bernard Desoize, Claudie Madoulet
Dec 18, 2002·Cell Biology and Toxicology·D Khynriam, S B Prasad
Jun 21, 2003·The Journal of Biological Chemistry·Xianhe BaiJack L Arbiser
Jul 3, 2004·Nature Reviews. Molecular Cell Biology·Julius J JeffersonRonald K H Liem
Dec 21, 2004·Critical Reviews in Oncology/hematology·Stephen G ChaneyYibing Wu
Dec 27, 2007·Acta Pharmacologica Sinica·Byung Hun Kim, Jae Youl Cho
Apr 9, 2008·Current Biology : CB·Fernando Martín-BelmonteKeith Mostov
Jul 22, 2008·Biochemical Pharmacology·Sergey DikalovJack L Arbiser
Aug 19, 2008·Biochimica Et Biophysica Acta·Ben N G Giepmans, Sven C D van Ijzendoorn
Sep 30, 2008·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Danyelle M TownsendMarie H Hanigan
Sep 30, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jui-Lung ShenYung-Hsiang Chen
Oct 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Melissa E MunroeGail A Bishop
Nov 10, 2011·Toxins·Ronald P MillerWilliam Brian Reeves
Jan 25, 2012·Journal of Veterinary Internal Medicine·S ChakrabartiJ Elliott
Mar 31, 2012·American Journal of Physiology. Cell Physiology·Dennis BrownHua A J Lu
Sep 28, 2012·American Journal of Physiology. Cell Physiology·Naofumi YuiDennis Brown
Dec 27, 2012·Current Opinion in Cell Biology·R Dyche Mullins, Scott D Hansen
Jul 24, 2014·Toxicology Mechanisms and Methods·Joyce TrujilloJosé Pedraza-Chaverri
Jul 25, 2014·European Journal of Pharmacology·Shaloam Dasari, Paul Bernard Tchounwou
Aug 29, 2014·BioMed Research International·Abdullah Ozkok, Charles L Edelstein
Nov 26, 2014·Reproductive Biology and Endocrinology : RB&E·Ashok AgarwalStefan S du Plessis
Jan 24, 2015·Redox Biology·Angara SureshbabuMary E Choi
Mar 1, 2015·Pharmacology, Biochemistry, and Behavior·Kunjbihari SulakhiyaNaba K Hazarika
Jun 24, 2015·Toxicology Letters·Takatoshi Karasawa, Peter S Steyger
Jun 19, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·David J WagnerJoanne Wang
Sep 17, 2016·International Journal of Oncology·Shujie ChengDaniel Sliva
Jul 1, 2017·BMC Veterinary Research·Yu-Wen KuoPei-Shiue Jason Tsai

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Citations

Apr 14, 2020·Journal of Cellular and Molecular Medicine·Ming LiTan-Qi Lou
Sep 27, 2020·Journal of the Formosan Medical Association = Taiwan Yi Zhi·Kuo-Chou ChiuTzong-Ming Shieh
May 15, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chen GaoZhihui Hao
Jul 13, 2021·Chinese Journal of Natural Medicines·Cheng ChenLi-Gen Lin
Aug 8, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Abdur RaufMohammad Ali Shariati

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Methods Mentioned

BETA
Protein Assay
Assay
confocal scanning microscopy

Software Mentioned

CellSens
ImageJ

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