Honokiol ameliorates oxidative stress-induced DNA damage and apoptosis of c2c12 myoblasts by ROS generation and mitochondrial pathway

Animal Cells and Systems
Cheol ParkYung Hyun Choi

Abstract

Honokiol is one of the main active components of Magnolia officinalis, and has been demonstrated to have multiple pharmacological activities against a variety of diseases. Recently, this phenolic compound is known to have antioxidant activity, but its mechanism of action remains unclear. The purpose of the current study was to evaluate the preventive effects of honokiol against oxidative stress-induced DNA damage and apoptosis in C2C12 myoblasts. The present study found that honokiol inhibited hydrogen peroxide (H2O2)-induced DNA damage and mitochondrial dysfunction, while reducing reactive oxygen species (ROS) formation. The inhibitory effect of honokiol on H2O2-induced apoptosis was associated with the up-regulation of Bcl-2 and down-regulation of Bax, thus reducing the Bax/Bcl-2 ratio that in turn protected the activation of caspase-9 and -3, and inhibition of poly (ADP-ribose) polymerase cleavage, which was associated with the blocking of cytochrome c release to the cytoplasm. Collectively, these results demonstrate that honokiol defends C2C12 myoblasts against H2O2-induced DNA damage and apoptosis, at least in part, by preventing mitochondrial-dependent pathway through scavenging excessive ROS.

References

Sep 1, 2006·American Journal of Physiology. Cell Physiology·Asa B Gustafsson, Roberta A Gottlieb
Jul 24, 2010·Antioxidants & Redox Signaling·Michel RigouletAnne Devin
Feb 1, 2011·Pharmacology & Therapeutics·Young-Jung LeeJin Tae Hong
Dec 20, 2011·Cellular and Molecular Life Sciences : CMLS·A V KulikovB Zhivotovsky
Nov 6, 2012·Ageing Research Reviews·Venus SosaMatilde E LLeonart
Mar 13, 2014·Current Pharmaceutical Design·Teresa Valero
Jun 5, 2014·Current Molecular Medicine·D Vara, G Pula
Dec 5, 2015·Frontiers in Physiology·Li Zuo, Benjamin K Pannell
Apr 10, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yağmur KirazYusuf Baran
May 18, 2016·Free Radical Biology & Medicine·George G RodneyReem Abo-Zahrah
Feb 22, 2017·Free Radical Biology & Medicine·Jaakko L Pohjoismäki, Steffi Goffart
Apr 27, 2017·Toxicological Research·Hye Lyun JeonSoo Jung Sohn
May 4, 2017·Immunological Reviews·Bart Tummers, Douglas R Green
Oct 21, 2017·The Journal of Nutritional Biochemistry·Satoshi HaramizuStephen E Alway
Feb 22, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Brittany G Durgin, Adam C Straub
Apr 5, 2018·Frontiers in Physiology·Yunfang GaoNandu Goswami
May 23, 2018·Oxidative Medicine and Cellular Longevity·Johanna ÁbrigoClaudio Cabello-Verrugio

❮ Previous
Next ❯

Citations

Jul 1, 2020·International Journal of Molecular Sciences·Ekaterina ProshkinaAlexey Moskalev

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
flow cytometry

Software Mentioned

CometScore
Cell Quest Pro

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis