Honokiol inhibits HepG2 migration via down-regulation of IQGAP1 expression discovered by a quantitative pharmaceutical proteomic analysis

Proteomics
Shufang LiangLijuan Chen

Abstract

Honokiol (HNK), a natural small molecular product, inhibited proliferation of HepG2 cells and exhibited anti-tumor activity in nude mice. In this article, we applied a novel sensitive stable isotope labeling with amino acids in cell culture-based quantitative proteomic method and a model of nude mice to investigate the correlation between HNK and the hotspot migration molecule Ras GTPase-activating-like protein (IQGAP1). The quantitative proteomic analysis showed that IQGAP1 was 0.53-fold down-regulated under 10 microg/mL HNK exposure for 24 h on HepG2 cells. Migration ability of HepG2 cells under HNK treatment was correlated with its expression level of IQGAP1. In addition, the biochemical validation on HepG2 cells and the tumor xenograft model further demonstrated that HNK decreased the expression level of IQGAP1 and its upstream proteins Cdc42/Rac1. These data supported that HNK can modulate cell adhesion and cell migration by acting on Cdc42/Rac1 signaling via IQGAP1 interactions with its upstream Cdc42/Rac1 proteins, which is a new molecular mechanism of HNK to exert its anti-tumor activity.

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Citations

Apr 9, 2016·International Journal of Biological Macromolecules·Shaowei WuRana Muhammad Aadil
Nov 17, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Yang WangQing-Yu He
Jul 28, 2019·Cell Communication and Signaling : CCS·Yanfang YangShufang Liang
Feb 15, 2017·BioMed Research International·Ruth Guillermo-LagaeChandradhar Dwivedi
Aug 28, 2014·Cancer Prevention Research·Faya M MillimounoXiaomeng Li
Jul 13, 2021·Chinese Journal of Natural Medicines·Cheng ChenLi-Gen Lin

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