Hop/Sti1 - A Two-Faced Cochaperone Involved in Pattern Recognition Receptor Maturation and Viral Infection

Frontiers in Plant Science
Christian E LammUwe Sonnewald

Abstract

Perception of pathogens by host pattern recognition receptors (PRRs) or R proteins is a prerequisite to promote successful immune responses. The Hsp70/Hsp90 organizing protein Hop/Sti1, a multifunctional cochaperone, has been implicated in the maturation of a receptor-like kinase (RLK) necessary for chitin sensing. However, it remains unknown whether Hop/Sti1 is generally participating in PRR genesis. Using RNA-interference (RNAi), we silenced Hop/Sti1 expression in Nicotiana tabacum to gain further insight into the role of the cochaperone in plant defense responses. As expected, transgenic plants do not respond to chitin treatment anymore. In contrast to this, trafficking and functionality of the flagellin PRR FLS2 were unaltered, suggesting a selective involvement of Hop/Sti1 during PRR maturation. Furthermore, Hop/Sti1 was identified as a cellular determinant of Potato virus Y (PVY) symptom development in tobacco, since PVY was able to accumulate to near wild-type level without provoking the usual veinal necrosis phenotype. In addition, typical antiviral host defense responses were suppressed in the transgenic plants. These data suggest that perception of PVY is dependent on Hop/Sti1-mediated receptor maturation, while viral...Continue Reading

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Citations

May 10, 2018·Plant, Cell & Environment·Nuria Fernández-BautistaM Mar Castellano
Jun 5, 2018·The Plant Journal : for Cell and Molecular Biology·Vincent MichelSylvie German-Retana
Apr 4, 2019·Molecular Plant Pathology·Annette Niehl, Manfred Heinlein
Dec 28, 2018·The Journal of Cell Biology·Aykut TuranChristiane Silke Heilingloh
Sep 26, 2020·Frontiers in Genetics·Shaloo MeenaParamjit Khurana
Nov 17, 2020·Frontiers in Plant Science·René ToribioM Mar Castellano

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Methods Mentioned

BETA
protein folding
transgenic
PCR
electrophoresis
Enzyme-Linked Immunosorbent Assay
ELISA
reverse-transcription PCR
glycosylation

Software Mentioned

ImageJ
SPSS Statistics
Excel
Stratagene MxPro

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