HOPX Defines Heterogeneity of Postnatal Subventricular Zone Neural Stem Cells

Stem Cell Reports
Stefan ZweifelOlivier Raineteau

Abstract

The largest diversity of neural lineages generated from the subventricular zone (SVZ) occurs early after birth and is regulated in a spatiotemporal manner depending on the expression of specific transcriptional cues. Transcriptomics and fate-mapping approaches were employed to explore the relationship between regional expression of transcription factors by neural stem cells (NSCs) and the specification of distinct neural lineages. Our results support an early priming of NSCs for the genesis of defined cell types depending on their spatial location in the SVZ and identify HOPX as a marker of a subpopulation primed toward astrocytic fates. Manipulation of HOPX expression, however, showed no effect on astrogenesis but resulted in marked changes in the number of NSCs and of their progenies. Taken together, our results highlight transcriptional and spatial heterogeneity of postnatal NSCs and reveal a key role for HOPX in controlling SVZ germinal activity.

Citations

Jan 24, 2019·International Journal of Molecular Sciences·Felix BeyerPatrick Küry
May 15, 2020·Nature Communications·Noreen EderSila K Ultanir
Jul 3, 2020·International Journal of Molecular Sciences·Samir Vaid, Wieland B Huttner
Dec 23, 2020·Nature Neuroscience·Sara BottesSebastian Jessberger
Dec 18, 2020·Frontiers in Oncology·Ángela Fontán-LozanoEsperanza R Matarredona
Jun 8, 2021·Current Opinion in Cell Biology·Anneline Pinson, Wieland B Huttner
Apr 13, 2021·The Journal of Investigative Dermatology·Olesya PavlovaDaniel Hohl

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Datasets Mentioned

BETA
GSE60905
GSE9566

Methods Mentioned

BETA
fluorescence-activated cell sorting
transgenic

Software Mentioned

LAS X
dmEPO
ImageJ
Photoshop
Excel
GraphPad Prism
dlEPO

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