Horizontal transfer of Shiga toxin and antibiotic resistance genes among Escherichia coli strains in house fly (Diptera: Muscidae) gut

Journal of Medical Entomology
M PetridisE Walker

Abstract

Whether the house fly, Musca domestica L., gut is a permissive environment for horizontal transfer of antibiotic resistance and virulence genes between strains of Escherichia coli is not known. House flies were immobilized and force fed suspensions of defined, donor strains of E. coli containing chloramphenicol resistance genes on a plasmid, or lysogenic, bacteriophage-born Shiga toxin gene stx1 (bacteriophage H-19B::Ap1). Recipient strains were E. coli lacking these mobile elements and genes but having rifampicin as a selectable marker. Plasmid transfer occurred at rates of 10(-2) per donor cell in the fly midgut and 10(-3) in the fly crop after 1 h of incubation postfeeding. Bacteriophage transfer rate was approximately 10(-6) per donor cell without induction, but induction with mitomycin C increased rates of transfer to 10(-2) per donor cell. These findings show that genes encoding antibiotic resistance or toxins will transfer horizontally among bacteria in the house fly gut via plasmid transfer or phage transduction. The house fly gut may provide a favorable environment for the evolution and emergence of pathogenic bacterial strains through acquisition of antibiotic resistance genes or virulence factors.

References

May 27, 1967·Nature·E Meynell, N Datta
Jul 1, 1983·Applied and Environmental Microbiology·P EcheverriaA McFarland
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·F C FranklinK N Timmis
Nov 29, 1996·Cell·E A Groisman, H Ochman
Jun 1, 1997·Journal of Clinical Microbiology·P GrübelD R Cave
Jan 1, 1997·Advances in Applied Microbiology·X Yin, G Stotzky
Mar 11, 1998·Microbiology·Jerneja AmbrožKičKDarja Žgur-Bertok
Apr 29, 1998·Current Microbiology·J E Urban, A Broce
Jun 2, 1998·The Journal of Infectious Diseases·P FengT S Whittam
Aug 26, 1998·Infection and Immunity·D W AchesonM K Waldor
Sep 14, 1999·Medical and Veterinary Entomology·K MoriyaH Watanabe
Nov 5, 1999·The American Journal of Tropical Medicine and Hygiene·M KobayashiN Agui
Mar 10, 2001·The Journal of Clinical Investigation·M S Donnenberg, T S Whittam
Aug 11, 2001·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D G Cvitkovitch
Apr 4, 2002·Oral Microbiology and Immunology·B Y WangH K Kuramitsu
Oct 31, 2002·Molecular Microbiology·B Joseph HinnebuschElisabeth Carniel
Nov 16, 2002·Current Opinion in Genetics & Development·Thomas S Whittam, Alyssa C Bumbaugh
Apr 28, 2004·Trends in Genetics : TIG·Olga ZhaxybayevaJ Peter Gogarten
May 20, 2004·International Journal of Medical Microbiology : IJMM·Jörg HackerUlrich Dobrindt
May 29, 2004·Genetics·Paul E Turner
Jun 23, 2004·Research in Microbiology·Vincent Burrus, Matthew K Waldor
Oct 21, 2004·International Journal of Medical Microbiology : IJMM·Sylvia HeroldHerbert Schmidt

❮ Previous
Next ❯

Citations

Jan 16, 2013·Annual Review of Entomology·John G Stoffolano, Aaron T Haselton
Apr 3, 2007·Applied and Environmental Microbiology·Martina BielaszewskaHelge Karch
Nov 6, 2007·Applied and Environmental Microbiology·Alexander MellmannMartina Bielaszewska
Sep 3, 2013·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·William A BryantJohn W Pinney
Nov 30, 2011·Future Microbiology·Veronica Casas, Stanley Maloy
May 11, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Masaru UsuiYutaka Tamura
Dec 20, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Akira FukudaYutaka Tamura
Jun 11, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Masaru UsuiYutaka Tamura
Apr 8, 2014·Applied and Environmental Microbiology·Ludek Zurek, Anuradha Ghosh
May 30, 2008·FEMS Microbiology Ecology·Frances R SlaterSarah L Turner
Jan 31, 2020·Food Safety·Akira FukudaYutaka Tamura
Jun 27, 2019·Microorganisms·Susanne A KraemerGabriel G Perron
Jul 1, 2014·Annals of the Entomological Society of America·John G StoffolanoLynn Tran
Jun 10, 2021·Trends in Microbiology·Katherine M ElstonJeffrey E Barrick

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.