Hormonal control by A-factor of morphological development and secondary metabolism in Streptomyces

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
Sueharu Horinouchi, T Beppu

Abstract

Streptomyces griseus, a well-known industrial producer of streptomycin, is a member of the genus Streptomyces, which shows a complex life cycle resembling that of fungi. A-factor, a C13 γ-butyrolactone compound, was discovered as a self-regulatory factor or a bacterial hormone to induce morphological differentiation and production of secondary metabolites, including streptomycin, in this organism. Accumulating evidence has revealed an A-factor-triggered signal cascade, which is composed of several key steps or components. These include: (i) AfsA catalyzing a crucial step of A-factor biosynthesis, (ii) the A-factor-specific receptor (ArpA), which acts as a transcriptional repressor for adpA, (iii) adpA, a sole target of ArpA, which encodes a global transcriptional activator AdpA, and (iv) a variety of members of the AdpA regulon, a set of the genes regulated by AdpA. A-factor is biosynthesized via five reaction steps, in which AfsA catalyzes acyl transfer between a β-ketoacyl-acyl carrier protein and the hydroxyl group of dihydroxyacetone phosphate. The receptor ArpA, belonging to the TetR family, is a homodimer, each subunit of which contains a helix-turn-helix DNA-binding motif and an A-factor-binding pocket. The three-dimensi...Continue Reading

Citations

Jan 27, 2019·Applied and Environmental Microbiology·Xu-Liang BuJun Xu
May 1, 2019·Journal of Bacteriology·Tomohiro KimuraYasuo Ohnishi
Jan 11, 2020·Frontiers in Microbiology·Dekun KongGuoqing Niu

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Methods Mentioned

BETA
SELEX
PCR
gel mobility

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