Hormonal control of rat adrenal phenylethanolamine N-methyltransferase. Enzyme activity, the final critical pathway

Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology
D L WongW Wang

Abstract

To examine whether glucocorticoids control rat adrenal phenylethanolamine N-methyltransferase (PNMT) through gene transcription, the effects of hypophysectomy and acute and chronic glucocorticoid replacement on PNMT mRNA and enzymatic activity were determined. Glucocorticoid depletion through hypophysectomy did not alter PNMT mRNA, whereas PNMT activity declined to approximately 25% of normal. A single dose of ACTH (4 IU SC) rapidly induced PNMT mRNA, with a six-fold peak at 6 hours postinjection. The short-term rise in PNMT mRNA was accompanied by an increase in corticosterone and elevated levels of glucocorticoid receptor mRNA. Ribosomal loading experiments suggested that available PNMT mRNA was fully utilized for protein synthesis. However, PNMT activity did not increase commensurately. Chronic ACTH treatment (4 IU SC daily for 7 days) sustained elevated levels of glucocorticoid receptor mRNA but returned corticosterone to hypophysectomized levels and decreased PNMT mRNA to 50% of normal. Despite the decline in PNMT mRNA and its partial utilization for protein synthesis, PNMT enzymatic activity was fully restored. These findings indicate that glucocorticoids exert marked but complex influences on PNMT gene transcription. In ...Continue Reading

Citations

Apr 29, 2006·Cellular and Molecular Neurobiology·Dona Lee Wong
Nov 4, 2010·Cellular and Molecular Neurobiology·Dona Lee WongRichard Kvetnansky
Jun 9, 2009·Shock·Rana I Sharara-ChamiJoseph A Majzoub
Sep 4, 2004·Neuroendocrinology·Larissa A PohoreckyRichard Kvetnansky
Oct 19, 2011·Oxidative Medicine and Cellular Longevity·James A G CrispoT C Tai
May 20, 1998·In Vitro Cellular & Developmental Biology. Animal·P I LelkesB R Unsworth
Jul 9, 2004·Annals of the New York Academy of Sciences·Dona L WongRichard Kvetnansky
Aug 2, 2013·Anais Da Academia Brasileira De Ciências·Ljubica GavrilovićSladjana Dronjak
May 22, 2002·Molecular Pharmacology·T C TaiDona L Wong
Jun 15, 2019·International Journal of Molecular Sciences·Carla L BuscetiFrancesco Fornai
Jan 18, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Anna VavřínováJosef Zicha
Jan 15, 2021·Anesthesia and Pain Medicine·Kwon Hui Seo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.