Hormonal control of reversible translocation of perilipin B to the plasma membrane in primary human adipocytes

The Journal of Biological Chemistry
Nabila AboulaichPeter Strålfors

Abstract

In adipocytes, perilipin coats and protects the central lipid droplet, which stores triacylglycerol. Alternative mRNA splicing gives rise to perilipin A and B. Hormones such as catecholamines and insulin regulate triacylglycerol metabolism through reversible serine phosphorylation of perilipin A. It was recently shown that perilipin was also located in triacylglycerol-synthesizing caveolae of the plasma membrane. We now report that perilipin at the plasma membrane of primary human adipocytes was phosphorylated on a cluster of threonine residues (299, 301, and 306) within an acidic domain that forms part of the lipid targeting domain. Perilipin B comprised <10% of total perilipin but was the major isoform associated with the plasma membrane of human adipocytes. This association was controlled by insulin and catecholamine: perilipin B was specifically depleted from the plasma membrane in response to the catecholamine isoproterenol, while insulin increased the amount of threonine phosphorylated perilipin at the plasma membrane. The reversible translocation of perilipin B to and from the plasma membrane in response to insulin and isoproterenol, respectively, suggests a specific function for perilipin B to protect newly synthesized ...Continue Reading

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Citations

Oct 14, 2008·Genes & Nutrition·Yula Sambuy
Aug 3, 2012·Journal of Proteome Research·Renee WangFrançois Fagotto
Apr 5, 2011·Journal of Molecular Cell Biology·Günter Müller
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Oct 16, 2007·Trends in Endocrinology and Metabolism : TEM·Unn OrtegrenPeter Strålfors
Oct 10, 2006·Biochemical and Biophysical Research Communications·Nabila AboulaichPeter Strålfors
Feb 28, 2015·European Journal of Medical Genetics·Musharraf JelaniJumana Yousuf Al-Aama
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Aug 21, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ruth C R MeexMatthijs K C Hesselink

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