Hormonal inhibition of endocytosis: novel roles for noradrenaline and G protein G(z).

The Journal of Physiology
Ying ZhaoGeoffrey W G Sharp

Abstract

The modulation of endocytosis following exocytosis by noradrenaline (NA), a physiological inhibitor of insulin secretion, was investigated in INS 832/13 cells using patch-clamp capacitance measurements. Endocytosis was inhibited by NA in a pertussis toxin-insensitive manner. Dialysing a synthetic peptide mimicking the C-terminus of the α-subunit of G(z) into the cells blocked the inhibition of endocytosis by NA. Cell-attached capacitance measurements indicated that inhibition by NA was due to a decreased number of endocytotic events without a change in vesicle size. Analysis of fission pore closure kinetics revealed two distinct fission modes, with NA selectively inhibiting the rapid fission pore closure events. Comparison of the actions of NA and deltamethrin, a calcineurin antagonist and potent inhibitor of endocytosis, demonstrated that they inhibit endocytosis by different mechanisms. These findings establish novel actions for NA and G(z) in insulin-secreting cells and possibly other cell types.

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Citations

Apr 12, 2012·American Journal of Physiology. Cell Physiology·Susanne G Straub, Geoffrey W G Sharp
Jun 21, 2014·Experimental & Molecular Medicine·Michelle E KimplePatrick J Casey
Sep 29, 2015·The Journal of Clinical Investigation·Fan FanXuelin Lou
Apr 14, 2016·The Journal of Endocrinology·Ying ZhaoGeoffrey W G Sharp
Jan 24, 2018·Molecular and Cellular Endocrinology·Amy C KellySean W Limesand
Aug 8, 2021·Proceedings of the National Academy of Sciences of the United States of America·Fan FanXuelin Lou

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