PMID: 8611636Feb 2, 1996Paper

Hormonal modulation of c-fos expression in isolated hepatocytes. Effects of angiotensin II and phorbol myristate acetate on transcription and mRNA degradation

Biochimica Et Biophysica Acta
C González-Espinosa, J A García-Sáinz

Abstract

It has been shown that angiotensin II and PMA increase the expression of proto-oncogenes (c-fos, c-myc and c-mos) in liver cells. In this study the effects of angiotensin II and PMA on c-fos transcription and mRNA stability were investigated. Using nuclear run-off transcription assays, it was observed that PMA and angiotensin II induced a rapid increase in c-fos transcription. The transcription rate of the GAPDH gene did not change, indicating that the effects were not general on gene transcription. The ability of these agents to modulate proto-oncogene mRNA stability was tested by measuring c-fos mRNA half-life. It was observed that c-fos mRNA half-life was relatively short (approximately 14-18 min) and that angiotensin II and PMA markedly stabilized mRNA, increasing its half-life (approximately 4-fold and approximately 2-fold, respectively). The protein synthesis inhibitor cycloheximide increased mRNA stability to a much greater extent. Our results clearly demonstrate that angiotensin II and PMA increased c-fos mRNA accumulation in liver cells through two actions: induction of c-fos gene transcription and increase in mRNA stability.

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W R HuckleH S Earp
Sep 9, 1992·Biochimica Et Biophysica Acta·C González-Espinosa, J A García-Sáinz
Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
Feb 28, 1991·Nature·L C Mahadevan, D R Edwards
Oct 30, 1990·Biochemical and Biophysical Research Communications·J A García-Sáinz, M Macías-Silva
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May 20, 1988·Science·F J RauscherB R Franza

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Citations

Apr 21, 1998·European Journal of Pharmacology·J A García-SáinzJ Vázquez-Prado
Jan 5, 1999·European Journal of Pharmacology·J A García-SáinzC González-Espinosa
May 10, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alla Amcheslavsky, Zvi Bar-Shavit
Apr 17, 2012·Biochemical and Biophysical Research Communications·John ØdegårdThoralf Christoffersen
Mar 14, 2000·Archives of Medical Research·J A García-SáinzR Villalobos-Molina

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