Hormonal regulation and characterization of MHG30 gene, a desaturase-like gene of hamster harderian gland

The Journal of Steroid Biochemistry and Molecular Biology
T EspositoB Varriale

Abstract

The harderian gland (HG) is an orbital gland of the vast majority of land vertebrates. In the Syrian hamster these glands display a marked sexual dimorphism. Here we present data on a male specific clone named MHG30. The MHG30 cDNA (1470 bp) has significant sequence homologies with human #15μ10#Δ6-desaturase enzymes. The expression of MHG30 has been found in male HG and in the liver of both sexes, no other tissue showing the presence of MHG30 mRNA. Castration brings the MHG30 levels below detectable level in about 7 days. In in vitro cultures of male hamster HG cells, androgens (A) determine an enhancement of MHG30 expression in a time-dependent manner. Conversely, a continuous decrement has been observed in control cells and in cells treated with A plus flutamide (F) or with A and cycloheximide (Cy). Incubation of cells in cultures supplemented with desamethason (Dex) or thyroid hormone (T3) also increases MHG30 expression while 17β-estradiol prevents the stimulatory effect exerted by A, Dex and T3. Findings strongly suggest that the MHG30 gene could be involved in supporting the sexual dimorphism and its expression is likely triggered by a series of hormonal interactions.

References

Apr 1, 1991·Molecular Endocrinology·N J CharestF S French
Sep 1, 1981·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·T Sakai
Dec 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·B Varriale, I Serino
Dec 1, 1994·Molecular and Cellular Endocrinology·P DomínguezA Menéndez-Peláez
Aug 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·B Varriale, P Chieffi
Apr 25, 2000·The Journal of Steroid Biochemistry and Molecular Biology·A CardoneB Varriale
May 3, 2005·The Journal of Steroid Biochemistry and Molecular Biology·Bruno Varriale, Teresa Esposito
Oct 14, 2008·The Journal of Steroid Biochemistry and Molecular Biology·Teresa EspositoBruno Varriale

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