Hormonal regulation and differential expression of neuropilin (NRP)-1 and NRP-2 genes in bovine granulosa cells

Reproduction : the Official Journal of the Society for the Study of Fertility
Takashi ShimizuAkio Miyamoto

Abstract

Although much is known about the biology of vascular endothelial growth factor (VEGF) and its receptors, little is known about the role of the VEGF receptors neuropilin (NRP)-1 and NRP-2 in the process of bovine follicle development. The aim of the present study was to examine the hormonal regulation of NRP-1 and NRP-2 mRNAs by real-time PCR in follicles from the bovine ovary and in cultured granulosa cells. The NRP-1 gene was expressed in the granulosa and theca cells in the post-selection (POF) and pre-selection (PRF) follicles in the bovine ovary. In contrast, the NRP-2 gene was expressed only in the theca cells in the POF and the PRF. The level of NRP-1 mRNA was significantly increased by treatment of the cultured granulosa cells with 10 ng/ml estradiol (E2). In contrast, the addition of progesterone (P4) to the culture medium decreased the expression of the NRP-1 gene. The level of NRP-1 mRNA was increased by 10 ng/ml E2 with or without 1 ng/ml P4, but the level of NRP-1 mRNA was decreased if the P4 level was increased to 10 ng/ml, even when 1 ng/ml E2 was also added. Follicle-stimulating hormone did not stimulate the expression of the NRP-1 gene. These results are the first data showing that NRP-1, but not NRP-2, is expre...Continue Reading

References

Aug 22, 2001·Biology of Reproduction·O J GintherK Kot
Jun 10, 2005·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Kanako MiyabayashiEimei Sato

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Citations

Dec 8, 2011·Endocrinology·Kyle C CairesDerek J McLean
Sep 30, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·R S RobinsonG E Mann
Oct 19, 2010·Journal of Assisted Reproduction and Genetics·Mourad AssidiMarc-André Sirard
Nov 23, 2011·Biochemical Society Transactions·Carolyn A Staton
Nov 30, 2018·Endocrine Reviews·Diane M DuffyThomas E Curry
Mar 28, 2008·The Biochemical Journal·Caroline Pellet-ManyIan Zachary

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