Hormonal regulation of estrogen and progestin receptors in decidual cells

Biology of Reproduction
W W Leavitt, A Takeda

Abstract

Total estrogen receptor (Re) and total progestin receptor (Rp) were measured in the cytosol and nuclear fractions from hamster deciduomal tissue and decidual cell cultures. Correlation of serum steroid (estradiol and progesterone) and deciduomal receptor profiles revealed a significant loss of Re during the first four days of decidualization that was not attributable to changes in serum steroid levels. A decidual cell-tissue culture system was used to study the receptor's recovery response to progesterone withdrawal. Decidual cells were plated and grown in Ham's F12/Dulbecco's modified Eagle's medium with 5% horse serum supplemented with insulin, transferrin, selenium and progesterone (10 ng/ml). Within 48 h of culture large, multinucleate decidual cells were observed by phase microscopy. At 72 h of culture in medium containing progesterone, only Rp was detectable in decidual cells. Re was not detectable (less than 200 fmol/mg DNA) in either cytosol or nuclei from cells maintained in the presence of progesterone. However, when progesterone was deleted from the medium, cytosol Re recovered progressively from 8 h to 16 h of culture. Progesterone withdrawal also caused parallel increases in cytosol and nuclear Rp, and estradiol tr...Continue Reading

Citations

May 7, 1990·Molecular and Cellular Endocrinology·C L Clarke
Mar 25, 1996·Molecular and Cellular Endocrinology·A Salmi, F M Rutanen
Jan 1, 1991·Annals of the New York Academy of Sciences·C FlamigniE Di Cosmo
Sep 1, 1989·General and Comparative Endocrinology·M S Yu, S M Ho
Jul 4, 2013·Molecular Endocrinology·Hen PrizantStephen R Hammes
Dec 1, 1988·General and Comparative Endocrinology·K W Selcer, W W Leavitt

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