Hormonal regulation of human follicle-stimulating hormone-beta subunit gene expression: GnRH stimulation and GnRH-independent androgen inhibition

Neuroendocrinology
T R Kumar, M J Low

Abstract

Previous studies from our laboratory demonstrated that chronic testosterone administration to castrated transgenic mice suppressed human follicle-stimulating hormone-beta (FSH beta) mRNA levels transcribed from a human transgene to approximately 20% of control values. In the present study we used primary pituitary cultures prepared from the transgenic mice and in vivo experiments in hypogonadal (hpg) mice carrying the human transgene to assess the role of hypothalamic gonadotropin-releasing hormone (GnRH) in this inhibitory action. The levels of human FSH beta mRNA in monolayer cultures of pituitary cells were decreased by 24-hour treatments with 10 nM testosterone propionate or 5 alpha-dihydrotestosterone to 13 and 26% of control values, respectively, in the absence of GnRH. For the in vivo experiments we introduced the 10-kb human FSH beta transgene into the hpg genetic background by selective crossbreeding. Daily injections of 1 microgram GnRH for 14 days induced expression of the human FSH beta gene in male and female mice. Maximal effects were obtained by GnRH treatment of gonadectomized, hpg transgenic mice. Human FSH beta mRNA levels rose to approximately 4- or 10-fold that of control males and females, respectively. The...Continue Reading

Citations

Dec 5, 2003·Molecular and Cellular Endocrinology·T Rajendra KumarMartin M Matzuk
Jul 3, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Stephen J Winters, Joseph P Moore
Apr 21, 2010·Fertility and Sterility·Daniel J BernardPankaj Lamba
Jan 18, 2006·Molecular and Cellular Endocrinology·T Rajendra KumarMalcolm J Low
Oct 24, 2006·Molecular and Cellular Endocrinology·T Rajendra Kumar
Feb 14, 2018·Journal of Molecular Endocrinology·Nandana Das, T Rajendra Kumar
Sep 29, 2018·Minerva ginecologica·Kamila SzymańskaAdolfo Rivero-Müller
Feb 23, 2019·Frontiers in Endocrinology·Rosemary McDonaldT Rajendra Kumar
Dec 20, 2014·The Journal of Biological Chemistry·Huizhen WangT Rajendra Kumar
May 22, 2018·Best Practice & Research. Clinical Endocrinology & Metabolism·Sara Babcock GilbertT Rajendra Kumar

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