PMID: 9545024Apr 17, 1998Paper

Hormonal regulation of leptin mRNA expression and preadipocyte recruitment and differentiation in porcine primary cultures of S-V cells

Obesity Research
X L ChenG J Hausman

Abstract

The hormonal regulation of leptin mRNA expression and the association between leptin expression and adipocyte differentiation were examined in primary cultures of porcine S-V cells with Northern blot and immunocytochemical analysis. Seeding for 3 days with fetal bovine serum (FBS) with varying levels of dexamethasone (Dex) increased levels of leptin mRNA in a dose-dependent manner in parallel with increases in the proportion of preadipocytes (AD-3 positive cells; AD-3, a preadipocyte marker). Six-day treatment with 10 or 850 nM insulin after FBS+Dex treatment resulted in a similar increase in leptin mRNA expression and morphological differentiation. However, significantly lower levels of leptin mRNA and smaller fat cells were observed in cultures treated with 1 nM insulin or 10 nM insulin-like growth factor-I (IGF-I). Dex-induced increases in leptin mRNA levels and AD-3 cell numbers were blocked completely by the addition of transforming growth factor-beta (TGF-beta) to FBS+Dex-treated cultures. However TGF-beta significantly increased fat cell size and leptin mRNA expression when added to ITS (insulin, 850 nM; transferrin, 5 microg/ml; and selenium, 5 microg/mL) treated cultures during the lipid-filling stage. When added with ...Continue Reading

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Citations

Feb 26, 2000·European Journal of Obstetrics, Gynecology, and Reproductive Biology·C SchubringW Kiess
Sep 28, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·R L McNeelH J Mersmann
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Apr 30, 2013·Domestic Animal Endocrinology·S YonekuraH Aso
Jun 23, 2009·The Journal of Allergy and Clinical Immunology·Andreina BrunoMark Gjomarkaj
Oct 4, 2005·Domestic Animal Endocrinology·Shinichi YonekuraYoshiaki Obara
Dec 7, 2010·Molecular and Cellular Endocrinology·Kuei-Fang ChungMonika Ehrhart-Bornstein
Jun 15, 2014·Journal of Molecular Endocrinology·Jacqueline M WallaceClare L Adam
Nov 20, 2002·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·S MargeticR A Hill
Aug 5, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Michelle T Foster, Timothy J Bartness
Dec 18, 2003·Journal of Animal Science·T G Ramsay

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