Hormonal regulation of rat testicular arginine vasopressin receptors

Endocrinology
B G Kasson, T L Tuchel

Abstract

Previous studies have implicated arginine vasopressin (AVP) as a negative intratesticular modulator of androgen production. The present studies were undertaken to further define the testicular AVP system by investigating potential influences of pituitary hormones on testicular receptors for AVP. Binding of [3H]AVP to testicular membranes was time, temperature, and pH dependent, with optimum binding at 90 min, 22 C, and pH 8.0. Addition of divalent cations to the incubation mixture significantly increased binding by 4-, 10-, 15-, and 35-fold with Ca2+, Zn2+, Mg2+, and Mn2+, respectively. The influence of pituitary hormones on testicular AVP receptors was initially assessed by comparing binding parameters obtained by Scatchard analysis of saturation binding data between membranes prepared from intact or hypophysectomized rats. Testicular membranes from intact animals bound 66 +/- 2 fmol AVP/mg protein, whereas membranes from animals hypophysectomized 10 days previously showed a significantly lower binding capacity of 40 +/- 3 fmol AVP/mg protein; however, the affinity of the receptors for AVP did not differ between these two groups. A similar decrease was observed when binding was compared between purified Leydig cells isolated f...Continue Reading

Citations

Aug 1, 1991·Molecular and Cellular Endocrinology·A Tahri-JouteiG Pointis
Mar 4, 2000·Journal of Pharmaceutical and Biomedical Analysis·A CandolfiA C Harrington
Mar 4, 2000·Journal of Pharmaceutical and Biomedical Analysis·A CandolfiD L Massart
Apr 1, 1992·Baillière's Clinical Endocrinology and Metabolism·G Verhoeven
Aug 1, 1996·Journal of Autonomic Pharmacology·V Ralevic, G Burnstock

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