PMID: 6402631Feb 28, 1983Paper

Hormone effects on muscarinic cholinergic binding in bovine and rat sympathetic superior cervical ganglia

Life Sciences
P V Gejman, D P Cardinali

Abstract

Muscarinic receptors were assessed by [3H]-quinuclidinyl benzilate (QNB) binding in 900 xg supernatants of bovine superior cervical ganglia (SCG). At 30 degrees C half maximal binding was reached within 3 min and equilibrium within 30 min. Scatchard analysis revealed a single population of binding sites with dissociation constant (Kd) = 0.15 +/- 0.01 nM and site concentration (Bmax) = 101 +/- 4 fmoles/mg prot. Binding was specific for muscarinic drugs. Incubation of bovine SCG with different hormones (10(-7)M) indicated that LH, TRH and testosterone depressed significantly Bmax, and that prolactin decreased both Kd and Bmax of [3H] -QNB binding. Several other hormones tested (TSH, GH, FSH, LHRH, angiotensin II, bradykinin, melatonin, estradiol, thyroxine and triiodothyronine) did not affect QNB binding. Hormone effects were not due to a direct interference with radioligand binding to membrane. The injection of LH to orchidectomized rats depressed Bmax of SCG QNB binding without changing the Kd. These results suggest that muscarinic cholinergic neurotransmission in SCG may be affected by hormones.

References

Jan 1, 1979·Annual Review of Neuroscience·B S McEwenD W Pfaff
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H I Yamamura, S H Snyder
Dec 1, 1972·Journal of Neurochemistry·W S Wilson, J R Cooper
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M SokolovskyS Avissar
Jan 1, 1981·Journal of Neural Transmission·D P CardinaliP V Gejman
Dec 1, 1981·Journal of Autonomic Pharmacology·T A McCalden
Sep 1, 1981·Journal of the Autonomic Nervous System·P V GejmanV E Nahmod
Nov 1, 1981·Journal of Neurochemistry·R G Siman, W L Klein

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Citations

Aug 11, 1989·European Journal of Pharmacology·M GalvanC Schudt
Aug 9, 2002·European Journal of Pharmacology·Rebecca HallCharles H V Hoyle
Jul 26, 1984·The New England Journal of Medicine·N S NadiE S Gershon
Feb 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Marilina CasaisAna María Rastrilla
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·M CasaisA M Rastrilla
Sep 10, 2014·Reproduction, Fertility, and Development·Sandra S VallcanerasMarilina Casais

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