Hormone induced changes in lactase glycosylation in developing rat intestine.

Molecular and Cellular Biochemistry
Kamaljit Kaur ChaudhryAkhtar Mahmood

Abstract

Lactase exists in both soluble and membrane-bound forms in suckling rat intestine. The distribution of lactase and its glycosylated isoforms in response to thyroxine or cortisone administration has been studied in suckling rats. 75% of lactase activity was detected, associated with brush borders, compared to 24% in the soluble fraction of 8-day-old rats. Thyroxine treatment enhanced soluble lactase activity to 34%, whereas particulate fraction was reduced to 67% compared to controls. Cortisone administration reduced soluble lactase activity from 24% in controls to 12% with a concomitant increase in membrane-bound activity to 89%. Western blot analysis revealed lactase signal, corresponding to 220 kDa in both the soluble and membrane fractions, which corroborated the enzyme activity data. The elution pattern of papain solubilized lactase from agarose-Wheat Germ agglutinin, or Concanavalin A or Jacalin agglutinin columns was different in the suckling and adult rat intestines. Also the elution profile of lactase activity from agarose-lectin columns was modulated in cortisone, thyroxine, and insulin injected pups, which suggests differences in glycosylated isoforms of lactase under these conditions. These findings suggest the role ...Continue Reading

References

Oct 15, 1991·Biochemical and Biophysical Research Communications·K Y YehP R Holt
May 20, 1974·Biochimica Et Biophysica Acta·E Birkenmeier, D H Alpers
Jan 1, 1993·Archives of Biochemistry and Biophysics·A MahmoodD H Alpers
Feb 14, 2002·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dingwei DaiW Allan Walker
Jan 1, 1964·Analytical Biochemistry·A DAHLQVIST
Feb 15, 2008·Molecular and Cellular Biochemistry·Kamaljit Kaur ChaudhryAkhtar Mahmood

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