Hormone-induced sperm-release in the critically endangered Booroolong frog (Litoria booroolongensis ): effects of gonadotropin-releasing hormone and human chorionic gonadotropin

Conservation Physiology
Aimee J SillaPhillip G Byrne

Abstract

Research into the development of reproductive technologies for amphibians has increased in recent years due to the rapid decline of amphibian species globally. Reproductive technologies have great potential to overcome captive breeding failure and improve the propagation and genetic management of threatened species. However, the incorporation of these technologies into conservation breeding programs has been protracted, primarily as a result of trial-and-error approaches to the refinement of hormone therapies. The present study investigated the effects of: (1) GnRH-a dose (0, 0.5, 1, 2, 4, 8 or 16 μg g-1), and (2) hCG dose (0, 2.5, 5, 10, 20 or 40 IU g-1), on the sperm-release response of the critically endangered Booroolong frog. Administration of GnRH-a at a dose of 0.5 μg g-1 resulted in the greatest number of sperm released (mean total sperm = 3.5 ×106, n = 11). Overall, hCG was more effective at eliciting spermiation in Booroolong frogs, with peak sperm release (mean total sperm = 25.1 ×106, n = 10) occurring in response to a dose of 40 IU g-1. Sperm output in response to 40 IU g-1 hCG was greatest between 1 and 6 h and steadily declined between 8 and 24 h post-hormone administration. Percent sperm motility peaked between ...Continue Reading

References

Jun 23, 2006·Reproductive Biology and Endocrinology : RB&E·Robert K BrowneAndrew Kouba
May 1, 2012·Reproduction, Fertility, and Development·V K UteshevE N Gakhova
Jan 15, 2015·Reproduction, Fertility, and Development·Caitlin E McDonoughAndrew J Kouba
Aug 27, 2015·Conservation Biology : the Journal of the Society for Conservation Biology·Gemma HardingLissette Pavajeau
Nov 26, 2015·Science Advances·Gerardo CeballosTodd M Palmer
Mar 26, 2016·General and Comparative Endocrinology·Maria Vu, Vance L Trudeau
Jun 11, 2017·Animal Reproduction Science·L M KeoghA J Silla
Apr 26, 2018·Reproduction, Fertility, and Development·Aimee J SillaPhillip G Byrne
Oct 26, 2018·Annual Review of Animal Biosciences·Aimee J Silla, Phillip G Byrne

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Methods Mentioned

BETA
urine collection
sperm collection

Software Mentioned

JMP Pro

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