Hormone-sensitive lipase is structurally related to acetylcholinesterase, bile salt-stimulated lipase, and several fungal lipases. Building of a three-dimensional model for the catalytic domain of hormone-sensitive lipase.

The Journal of Biological Chemistry
J A ContrerasCecilia Holm

Abstract

Hormone-sensitive lipase is the key enzyme in the mobilization of fatty acids from adipose tissue, thereby playing a crucial role in the overall energy homeostasis in mammals. Its activity is stimulated by catecholamines through cAMP-dependent phosphorylation of a single serine, a process that is prevented by insulin. This regulatory property is unique to this enzyme among all known lipases and has been acquired during evolution through insertion of a regulatory module into an ancestral lipase. Sequence alignments have failed to detect significant homology between hormone-sensitive lipase and the rest of the mammalian lipases and esterases, to which this enzyme is only very distantly related. In the present work, we report the finding of a remarkable secondary structure homology between hormone-sensitive lipase and the enzymes from a superfamily of esterases and lipases that includes acetylcholinesterase, bile salt-stimulated lipase, and several fungal lipases. This finding, based on the identification of the secondary structure elements in the hormone-sensitive lipase sequence, has allowed us to construct a three-dimensional model for the catalytic domain of hormone-sensitive lipase. The model reveals the topological organizat...Continue Reading

References

Apr 1, 1992·Protein Engineering·D L OllisJ Schrag
Sep 10, 1992·Nature·H van TilbeurghC Cambillau
Feb 22, 1990·Nature·F K WinklerW Hunziker
Jul 20, 1990·European Journal of Biochemistry·A J Garton, S J Yeaman
Apr 9, 1990·Biochimica Et Biophysica Acta·S J Yeaman
Mar 1, 1990·Journal of Molecular Graphics·G Vriend
Oct 14, 1987·Biochemical and Biophysical Research Communications·C HolmG Fredrikson
Sep 1, 1995·Trends in Biochemical Sciences·R A Sayle, E J Milner-White
Dec 1, 1993·Trends in Biochemical Sciences·D Langin, C Holm
Jan 1, 1994·Protein Science : a Publication of the Protein Society·P GrochulskiM Cygler
Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Jul 20, 1993·Journal of Molecular Biology·B Rost, C Sander
Mar 20, 1993·Journal of Molecular Biology·J D Schrag, M Cygler
Oct 15, 1996·The Biochemical Journal·T OsterlundC Holm

❮ Previous
Next ❯

Citations

Apr 24, 2009·Molecular and Cellular Biochemistry·Sarah J Annesley, Paul R Fisher
Apr 12, 2003·Progress in Lipid Research·Thierry Raclot
Sep 30, 1998·Current Opinion in Biotechnology·L M Kauvar, H O Villar
Jun 2, 2011·Chemical Reviews·Rosalind A Coleman, Douglas G Mashek
Apr 24, 2012·Chemical Reviews·Reem SmoumMorris Srebnik
Sep 24, 1999·The Biochemical Journal·J L Arpigny, K E Jaeger
Mar 30, 2001·European Journal of Biochemistry·T Osterlund
Sep 24, 1999·Protein Science : a Publication of the Protein Society·G MancoM Rossi
Jun 8, 2000·Annals of the New York Academy of Sciences·C LondosC M Rondinone
Aug 15, 2000·Annual Review of Nutrition·C HolmJ A Contreras
Jan 28, 2004·Annual Review of Pharmacology and Toxicology·Jacques RobidouxSheila Collins
Aug 4, 2005·BMC Bioinformatics·Fabrizio FerrèManuela Helmer-Citterich
Sep 10, 2005·Lipids in Health and Disease·G R SridharP Vijayalakshmi
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·W J ShenF B Kraemer
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J OsugaN Yamada
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·Y S KimB J Oh
Jan 11, 2005·Médecine sciences : M/S·Coralie SengenesMax Lafontan
Jan 19, 2011·Gene·Antonis D LampidonisDimitrios J Stravopodis
Jan 22, 2015·Meta Gene·Daniel Estanislao GoszczynskiGuillermo Giovambattista
Sep 7, 2007·Experimental Cell Research·Sari LonginVeerle Janssens
May 23, 2008·Chemico-biological Interactions·John E CasidaKazutoshi Fujioka
Apr 29, 2006·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Dominique Langin
Nov 23, 2010·Progress in Lipid Research·Achim LassRudolf Zechner
May 26, 2009·Progress in Lipid Research·Max Lafontan, Dominique Langin
May 20, 2004·Bioorganic & Medicinal Chemistry Letters·Derek B LoweKevin B Clairmont
Oct 5, 2002·Journal of Lipid Research·Fredric B Kraemer, Wen-Jun Shen
Oct 3, 2001·The Journal of Biological Chemistry·A S GreenbergF B Kraemer

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.