Hormone-sensitive lipase overexpression increases cholesteryl ester hydrolysis in macrophage foam cells

Arteriosclerosis, Thrombosis, and Vascular Biology
J L EscaryMichael C Schotz

Abstract

Atherosclerosis is a complex physiopathologic process initiated by the formation of cholesterol-rich lesions in the arterial wall. Macrophages play a crucial role in this process because they accumulate large amounts of cholesterol esters (CEs) to form the foam cells that initiate the formation of the lesion and participate actively in the development of the lesion. Therefore, prevention or reversal of CE accumulation in macrophage foam cells could result in protection from multiple pathological effects. In this report, we show that the CE hydrolysis catalyzed by neutral cholesterol ester hydrolase (nCEH) can be modulated by overexpression of hormone-sensitive lipase (HSL) in macrophage foam cells. For these studies, RAW 264.7 cells, a murine macrophage cell line, were found to be a suitable model of foam cell formation. HSL expression and nCEH activity in these cells and in peritoneal macrophages were comparable. In addition, antibody titration showed that essentially all nCEH activity in murine macrophages was accounted for by HSL. To examine the effect of HSL overexpression on foam cell formation, RAW 264.7 cells were stably transfected with a rat HSL cDNA. The resulting HSL overexpression increased hydrolysis of cellular CE...Continue Reading

References

Sep 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S K BasuM S Brown
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K S MoultonC K Glass
Sep 25, 1992·Proceedings of the National Academy of Sciences of the United States of America·J J EganC Londos
Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A M HenneyS Humphries
Jan 16, 1990·Biochimica Et Biophysica Acta·D I Goldberg, J C Khoo
Apr 9, 1990·Biochimica Et Biophysica Acta·S J Yeaman
Oct 25, 1988·Nucleic Acids Research·C HolmM C Schotz
Oct 14, 1987·Biochemical and Biophysical Research Communications·C HolmG Fredrikson
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·A HorvaiC K Glass
Apr 1, 1993·Immunology and Cell Biology·K J StaceyD A Hume
Aug 15, 1996·The Biochemical Journal·C A JepsonS J Yeaman
Oct 1, 1996·Current Opinion in Lipidology·P LibbyR T Lee

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Citations

Dec 4, 2003·Biochimica Et Biophysica Acta·Henry A ChoyMichael C Schotz
Oct 12, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·W J JohnsonD W Bernard
Sep 11, 2008·The Journal of Biological Chemistry·Hiroaki OkazakiShun Ishibashi
Aug 11, 2012·PloS One·Michitaka SuzukiToyoshi Fujimoto
Apr 7, 2011·Journal of Atherosclerosis and Thrombosis·Motohiro SekiyaShun Ishibashi
Aug 24, 2011·Journal of Electron Microscopy·Michitaka SuzukiToyoshi Fujimoto
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·A R Saltiel
Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J OsugaN Yamada
Aug 15, 2000·Annual Review of Nutrition·C HolmJ A Contreras
Oct 5, 2002·Journal of Lipid Research·Fredric B Kraemer, Wen-Jun Shen
Aug 30, 2002·The Journal of Biological Chemistry·Lisa O'RourkePeter R Shepherd
Dec 15, 2004·Journal of Cellular Biochemistry·Jin-Shan ChenSeu-Mei Wang
May 17, 2019·Cardiovascular Research·Laurent Yvan-CharvetGiuseppe Danilo Norata
Jul 3, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Michele R PlewesJohn S Davis
Apr 3, 2020·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Matteo TardelliMichael Trauner
Jul 4, 2003·Current Opinion in Lipidology·Guenter HaemmerleRudolf Zechner
Sep 27, 2019·Pharmacological Reviews·Dongdong WangAtanas G Atanasov
Apr 16, 2016·Molecular Medicine Reports·Janeesh Plakkal AyyappanYoung-Hwa Goo
Jan 3, 2021·Progress in Lipid Research·Emeline RecazensDominique Langin
Sep 3, 2009·Cell Metabolism·Motohiro SekiyaShun Ishibashi
Nov 3, 2009·Vascular Pharmacology·Shobha GhoshJingmei Song

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