Hormone synthesis and responsiveness of spontaneous granulosa cell tumors in (SWR x SWXJ-9) F1 mice

Gynecologic Oncology
P M GoczeD A Freeman

Abstract

Granulosa cell tumors spontaneously occur in approximately 10-25% of female (SWR x SWXJ-9) F1 mice. The present studies were designed to test whether tumor-bearing mice produce a distinct hormonal profile by which they could be identified and determine whether cultured tumor cells are responsive to hormones and growth factors that regulate normal granulosa cells. Samples of female mouse blood taken from age 3 to 10 weeks allowed estimation of serum FSH, 17beta-estradiol, and inhibin levels for normal mice and for mice destined to develop tumors. These studies indicated that FSH and 17beta-estradiol values differed between normal and tumor-bearing animals, but overlapped sufficiently that such values could not accurately predict the tumor-bearing state. Inhibin concentrations did differentiate normal from tumor-bearing animals in all cases. Increased levels of inhibin were observed coincident in time with visibly detectable tumors within the ovaries. Compared to inhibin synthesis in vivo, hormonal responsiveness in vitro was much more variable. Steroidogenesis was stimulated in all tumors by dibutyryl-cAMP and low-density lipoprotein (LDL). Some, but not all, tumors responded to IGF1, EGF, FSH, and hCG. In about one-half of the ...Continue Reading

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Citations

Nov 28, 2012·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Kerri N SmithAnn M Dorward
Sep 25, 2009·Endocrine Reviews·Mark A EdsonMartin M Matzuk
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·R A KeriJ H Nilson
Apr 28, 2007·Endocrine Reviews·Jung-Hye ChoiPeter C K Leung
Oct 28, 1998·Toxicologic Pathology·W G BeamerJ P Sundberg

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