PMID: 11916434Mar 28, 2002Paper

Horseradish peroxidase catalyzed nitric oxide formation from hydroxyurea

Journal of the American Chemical Society
Jinming HuangS Bruce King

Abstract

Hydroxyurea represents an approved treatment for sickle cell anemia and a number of cancers. Chemiluminescence and electron paramagnetic resonance spectroscopic studies show horseradish peroxidase catalyzes the formation of nitric oxide from hydroxyurea in the presence of hydrogen peroxide. Gas chromatographic headspace analysis and infrared spectroscopy also reveal the production of nitrous oxide in this reaction, which provides evidence for nitroxyl, the one-electron reduced form of nitric oxide. These reactions also generate carbon dioxide, ammonia, nitrite, and nitrate. None of these products form within 1 h in the absence of hydrogen peroxide or horseradish peroxidase. Electron paramagnetic resonance spectroscopy and trapping studies show the intermediacy of a nitroxide radical and a C-nitroso species during this reaction. Absorption spectroscopy indicates that both compounds I and II of horseradish peroxidase act as one-electron oxidants of hydroxyurea. Nitroxyl, generated from Angeli's salt, reacts with ferric horseradish peroxidase to produce a ferrous horseradish peroxidase-nitric oxide complex. Electron paramagnetic resonance experiments with a nitric oxide specific trap reveal that horseradish peroxidase is capable o...Continue Reading

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Citations

Mar 15, 2012·Analytical and Bioanalytical Chemistry·Dulan B GunasekaraJosé Alberto Fracassi da Silva
Aug 12, 2004·Free Radical Biology & Medicine·S Bruce King
Apr 15, 2004·Phytochemistry·Luis A del RíoJuan B Barroso
Oct 4, 2013·Journal of Chemical Information and Modeling·Sai Lakshmana VankayalaH Lee Woodcock
Jan 18, 2011·Antioxidants & Redox Signaling·Jenna F DuMond, S Bruce King
Jan 18, 2011·Antioxidants & Redox Signaling·Wilmarie Flores-SantanaDavid A Wink
Jan 18, 2003·The Journal of Clinical Investigation·S Bruce King
Nov 10, 2012·Free Radical Research·Andrey A BobkoValery V Khramtsov
Jan 15, 2013·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh GillNarendra Tuteja
Sep 6, 2011·Plant Science : an International Journal of Experimental Plant Biology·Andreas Fröhlich, Jörg Durner
Feb 20, 2007·Nitric Oxide : Biology and Chemistry·Vladan P CokicAlan N Schechter
Oct 13, 2009·Fundamental & Clinical Pharmacology·Hind SassiAnne Hulin
Apr 25, 2006·Free Radical Biology & Medicine·Jinming HuangS Bruce King
May 29, 2012·Biochimica Et Biophysica Acta·Yuval SamuniSara Goldstein
Apr 20, 2005·Bioorganic & Medicinal Chemistry Letters·Richard L PenningtonS Bruce King
Jan 12, 2005·European Journal of Medicinal Chemistry·Biljana NigovićKresimir Sanković
Dec 1, 2015·Dalton Transactions : an International Journal of Inorganic Chemistry·Harishchandra Subedi, Nicola E Brasch
Oct 23, 2018·Angewandte Chemie·Yelu Shi, Yong Zhang
May 27, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Julie A ReiszS Bruce King
May 8, 2010·Dalton Transactions : an International Journal of Inorganic Chemistry·Debabrata ChatterjeeRudi van Eldik
Dec 20, 2011·Organic & Biomolecular Chemistry·Ivana Vinković VrčekMladen Biruš
Jan 30, 2019·Experimental Biology and Medicine·Nicola Conran, Lidiane Torres
Oct 7, 2019·Physiologia Plantarum·Sheelavanta M ShivarajRupesh Deshmukh
Jan 12, 2021·Free Radical Research·Junichi FujiiMalay B Mukherjee
Jan 21, 2021·British Journal of Haematology·Betty S PaceAbdullah Kutlar
Aug 24, 2020·Free Radical Biology & Medicine·George T MukoseraArlin B Blood
May 28, 2010·Journal of the American Chemical Society·Uri SamuniSara Goldstein

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