Horseradish peroxidase: modulation of properties by chemical modification of protein and heme

Biochemistry. Biokhimii︠a︡
G S ZakharovaV I Tishkov

Abstract

Horseradish peroxidase (HRP) is one of the most studied enzymes of the plant peroxidase superfamily. HRP is also widely used in different bioanalytical applications and diagnostic kits. The methods of genetic engineering and protein design are now widely used to study the catalytic mechanism and to improve properties of the enzyme. Here we review the results of another approach to HRP modification-through the chemical modification of amino acids or prosthetic group of the enzyme. Computer models of HRPs with modified hemes are in good agreement with the experimental data.

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Citations

Feb 11, 2015·Biochimie·Amanda BernardesIgor Polikarpov
May 24, 2019·Bioscience, Biotechnology, and Biochemistry·Takaaki KojimaHideo Nakano
Apr 1, 2014·Pharmaceutical Bioprocessing·Oliver Spadiut, Christoph Herwig
Apr 2, 2019·PloS One·Sonali CorreaLorena Betancor
Oct 28, 2019·Biotechnology Journal·Shuting HanDario L Helbing

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