Hospital Epidemiology of Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Hospital in Moshi, Tanzania, as Determined by Whole Genome Sequencing

BioMed Research International
Happiness H KumburuFrank M Aarestrup

Abstract

To determine molecular epidemiology of methicillin-resistant S. aureus in Tanzania using whole genome sequencing. DNA from 33 Staphylococcus species was recovered from subcultured archived Staphylococcus isolates. Whole genome sequencing was performed on Illumina Miseq using paired-end 2 × 250 bp protocol. Raw sequence data were analyzed using online tools. Full susceptibility to vancomycin and chloramphenicol was observed. Thirteen isolates (43.3%) resisted cefoxitin and other antimicrobials tested. Multilocus sequence typing revealed 13 different sequence types among the 30 S. aureus isolates, with ST-8 (n = seven, 23%) being the most common. Gene detection in S. aureus stains were as follows: mecA, 10 (33.3%); pvl, 5 (16.7%); tst, 2 (6.7%). The SNP difference among the six Tanzanian ST-8 MRSA isolates ranged from 24 to 196 SNPs and from 16 to 446 SNPs when using the USA300_FPR3757 or the USA500_2395 as a reference, respectively. The mutation rate was 1.38 × 10-11 SNPs/site/year or 1.4 × 10-6 SNPs/site/year as estimated by USA300_FPR3757 or the USA500_2395, respectively. S. aureus isolates causing infections in hospitalized patients in Moshi are highly diverse and epidemiologically unrelated. Temporal phylogenetic analysis pr...Continue Reading

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Citations

Jul 11, 2019·BMC Infectious Diseases·Frederick K WangaiR Andrew Seaton
Feb 16, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Joel ManyahiBjørn Blomberg

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Datasets Mentioned

BETA
PRJEB23314

Software Mentioned

SAMTools
MLST
ResFinder
RecHMM
Bayesian Evolutionary Analysis Sampling Trees ( BEAST )
BWA
Tracer
STATA
BEAST
FastTree

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