Host- and pathogen-derived adjuvant coatings on protein nanoparticle vaccines

Bioengineering & Translational Medicine
Timothy Z ChangJulie A Champion

Abstract

Nanoparticulate and molecular adjuvants have shown great efficacy in enhancing immune responses, and the immunogenic vaccines of the future will most likely contain both. To investigate the immunostimulatory effects of molecular adjuvants on nanoparticle vaccines, we have designed ovalbumin (OVA) protein nanoparticles coated with two different adjuvants-flagellin (FliC) and immunoglobulin M (IgM). These proteins, derived from Salmonella and mice, respectively, are representatives of pathogen- and host-derived molecules that can enhance immune responses. FliC-coated OVA nanoparticles, soluble FliC (sFliC) admixed with OVA nanoparticles, IgM-coated nanoparticles, and OVA-coated nanoparticles were assessed for immunogenicity in an in vivo mouse immunization study. IgM coatings on nanoparticles significantly enhanced both antibody and T cell responses, and promoted IgG2a class switching but not affinity maturation. FliC-coated nanoparticles and FliC-admixed with nanoparticles both triggered IgG2a class switching, but only FliC-coated nanoparticles enhanced antibody affinity maturation. Our findings that affinity maturation and class switching can be directed independently of one another suggest that adjuvant coatings on nanoparticl...Continue Reading

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Citations

Oct 27, 2018·Expert Review of Vaccines·Ye WangBao-Zhong Wang
Jan 10, 2018·Bioengineering & Translational Medicine·Pankaj Karande
Jun 8, 2019·Annual Review of Chemical and Biomolecular Engineering·Alexandra N Tsoras, Julie A Champion
Jun 6, 2020·Analytical and Bioanalytical Chemistry·Karsten M PoulsenChristine K Payne
Mar 11, 2020·Frontiers in Immunology·Kyle SaylorChenming Zhang
Jun 30, 2019·Bioengineering & Translational Medicine·Yaqi LyuLifang Yin
Jan 29, 2020·Bioengineering & Translational Medicine·Krystina L Hess, Christopher M Jewell
Jan 30, 2018·Bioconjugate Chemistry·Bob J IgnacioMartijn Verdoes
Nov 6, 2018·ACS Infectious Diseases·Lei Deng, Bao-Zhong Wang

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Methods Mentioned

BETA
dynamic light scattering
ELISA
Assay
flow cytometry
dissection
biolayer interferometry
Biosensors

Software Mentioned

Octet Data Analysis
ImageQuant TL
Octet RED96
GraphPad Prism

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