Host acyl coenzyme A binding protein regulates replication complex assembly and activity of a positive-strand RNA virus.

Journal of Virology
Jiantao ZhangXiaofeng Wang

Abstract

All positive-strand RNA viruses reorganize host intracellular membranes to assemble their replication complexes. Similarly, brome mosaic virus (BMV) induces two alternate forms of membrane-bound RNA replication complexes: vesicular spherules and stacks of appressed double-membrane layers. The mechanisms by which these membrane rearrangements are induced, however, remain unclear. We report here that host ACB1-encoded acyl coenzyme A (acyl-CoA) binding protein (ACBP) is required for the assembly and activity of both BMV RNA replication complexes. ACBP is highly conserved among eukaryotes, specifically binds to long-chain fatty acyl-CoA, and promotes general lipid synthesis. Deleting ACB1 inhibited BMV RNA replication up to 30-fold and resulted in formation of spherules that were ∼50% smaller but ∼4-fold more abundant than those in wild-type (wt) cells, consistent with the idea that BMV 1a invaginates and maintains viral spherules by coating the inner spherule membrane. Furthermore, smaller and more frequent spherules were preferentially formed under conditions that induce layer formation in wt cells. Conversely, cellular karmella structures, which are arrays of endoplasmic reticulum (ER) membranes formed upon overexpression of ce...Continue Reading

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Citations

May 31, 2014·PLoS Computational Biology·Deborah ChasmanMark Craven
Nov 3, 2014·Annual Review of Virology·Jean-François Laliberté, Huanquan Zheng
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jiantao ZhangXiaofeng Wang
Nov 6, 2012·Current Opinion in Virology·Romain GrangeonJean-François Laliberté
Oct 23, 2012·Current Opinion in Virology·Akira Mine, Tetsuro Okuno
Feb 15, 2015·Medical Microbiology and Immunology·Elisabeth AmmerAndreas Henke
Jul 4, 2013·Current Opinion in Plant Biology·Peter PalukaitisJohn P Carr
May 25, 2012·Current Opinion in Microbiology·Arturo Diaz, Paul Ahlquist
Dec 3, 2014·Current Opinion in Virology·Arturo Diaz, Xiaofeng Wang
Sep 12, 2018·Viruses·Hernan Garcia-Ruiz
Mar 14, 2019·Frontiers in Microbiology·Zhenlu ZhangXiaofeng Wang
Oct 1, 2019·Annual Review of Virology·Ellen Ketter, Glenn Randall

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