Host airway proteins interact with Staphylococcus aureus during early pneumonia

Infection and Immunity
Christy L VenturaCraig E Rubens

Abstract

Staphylococcus aureus is a major cause of hospital-acquired pneumonia and is emerging as an important etiological agent of community-acquired pneumonia. Little is known about the specific host-pathogen interactions that occur when S. aureus first enters the airway. A shotgun proteomics approach was utilized to identify the airway proteins associated with S. aureus during the first 6 h of infection. Host proteins eluted from bacteria recovered from the airways of mice 30 min or 6 h following intranasal inoculation under anesthesia were subjected to liquid chromatography and tandem mass spectrometry. A total of 513 host proteins were associated with S. aureus 30 min and/or 6 h postinoculation. A majority of the identified proteins were host cytosolic proteins, suggesting that S. aureus was rapidly internalized by phagocytes in the airway and that significant host cell lysis occurred during early infection. In addition, extracellular matrix and secreted proteins, including fibronectin, antimicrobial peptides, and complement components, were associated with S. aureus at both time points. The interaction of 12 host proteins shown to bind to S. aureus in vitro was demonstrated in vivo for the first time. The association of hemoglobin...Continue Reading

References

May 18, 1999·The American Journal of Physiology·S J SkerrettC B Wilson
Jan 10, 2001·The Journal of Cell Biology·J GarinM Desjardins
Apr 16, 2002·Molecular Microbiology·John M Taylor, David E Heinrichs
Jun 14, 2002·The Biochemical Journal·Otilia V VieiraSergio Grinstein
Jun 14, 2002·FEBS Letters·Tomi MölkänenPentti Kuusela
Feb 8, 2003·Science·Sarkis K MazmanianOlaf Schneewind
Apr 2, 2003·Nature Reviews. Immunology·Michel Desjardins
Jan 1, 1964·The Journal of Experimental Medicine·G M GREEN, E H KASS
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
Feb 26, 2004·Molecular Microbiology·Simon R ClarkeSimon J Foster
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shawn J SkerrettChristopher B Wilson
May 12, 2004·FEMS Microbiology Letters·Joakim BjerketorpLars Frykberg
Jul 10, 2004·The Journal of Infectious Diseases·Lawrence Y L LeeEric L Brown
Sep 14, 2004·Science·Eric P SkaarOlaf Schneewind
Jan 3, 2006·Seminars in Respiratory and Critical Care Medicine·Suzanne F Bradley
Jan 13, 2006·Omics : a Journal of Integrative Biology·Roger HigdonEugene Kolker
Oct 3, 2006·Advances in Microbial Physiology·Simon R Clarke, Simon J Foster
Oct 17, 2006·Journal of Bacteriology·Victor J TorresEric P Skaar
Nov 15, 2006·Infection and Immunity·Juliane Bubeck WardenburgOlaf Schneewind
Nov 24, 2006·Bioinformatics·Roger Higdon, Eugene Kolker
Jan 20, 2007·Science·Maria Labandeira-ReyM Gabriela Bowden

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Citations

Oct 15, 2008·Infection and Immunity·Christy L VenturaCraig E Rubens
Dec 8, 2010·Proteomics. Clinical Applications·Henry J WindleDermot P Kelleher
Mar 1, 2013·Big Data·Roger HigdonEugene Kolker

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