Host biotin is required for liver stage development in malaria parasites

Proceedings of the National Academy of Sciences of the United States of America
Teegan A Dellibovi-RaghebSean T Prigge

Abstract

Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that is the target of several classes of herbicides. Malaria parasites contain a plant-like ACC, and this is the only protein predicted to be biotinylated in the parasite. We found that ACC is expressed in the apicoplast organelle in liver- and blood-stage malaria parasites; however, it is activated through biotinylation only in the liver stages. Consistent with this observation, deletion of the biotin ligase responsible for ACC biotinylation does not impede blood-stage growth, but results in late liver-stage developmental defects. Biotin depletion increases the severity of the developmental defects, demonstrating that parasite and host biotin metabolism are required for normal liver-stage progression. This finding may link the development of liver-stage malaria parasites to the nutritional status of the host, as neither the parasite nor the human host can synthesize biotin.

References

Jan 1, 1992·Progress in Lipid Research·D L CintiS K Suneja
Jan 1, 1989·Annual Review of Biochemistry·J R Knowles
Jan 1, 1983·Annual Review of Biochemistry·S J WakilV C Joshi
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·A León-Del-RioR A Gravel
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·R F WallerG I McFadden
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·J JelenskaP Gornicki
Dec 26, 2001·Biochimica Et Biophysica Acta·N Peeters, I Small
Jan 11, 2002·The Journal of Biological Chemistry·Laurence DenisClaude Alban
Jun 5, 2003·Archives of Biochemistry and Biophysics·Basil J NikolauEve Syrkin Wurtele
Jul 2, 2003·Acta Crystallographica. Section D, Biological Crystallography·Stephen P MuenchSean T Prigge
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Claude AlbanRoland Douce
Apr 15, 2004·Nature Reviews. Microbiology·Stuart A RalphGeoffrey I McFadden
Oct 7, 2004·Journal of Molecular Biology·Naresh SinghJohn H Adams
Feb 6, 2007·Trends in Parasitology·Sylke Müller, Barbara Kappes
Dec 11, 2008·Cellular Microbiology·Ashley M VaughanStefan H I Kappe
Mar 26, 2009·BioFactors·Janos ZempleniYousef I Hassan
May 13, 2010·International Review of Cell and Molecular Biology·Frank Seeber, Dominique Soldati-Favre
Jun 29, 2010·Bioorganic & Medicinal Chemistry Letters·Theola LouieKeith G Watson
Jan 5, 2011·PLoS Pathogens·Sebastian KehrKatja Becker
Jan 22, 2011·Molecular and Biochemical Parasitology·Suresh M GanesanAkhil B Vaidya
Dec 2, 2011·Cellular Microbiology·Christina DeschermeierVolker T Heussler
Dec 20, 2011·The Journal of Biological Chemistry·Srinivasan RamakrishnanBoris Striepen
Jan 10, 2012·International Journal for Parasitology·Katherine E JacksonStuart A Ralph
Aug 8, 2012·Cellular and Molecular Life Sciences : CMLS·Liang Tong
Mar 16, 2013·Cellular Microbiology·Brie FalkardDavid Fidock
Apr 17, 2013·Proceedings of the National Academy of Sciences of the United States of America·Cyrille Y BottéGeoffrey I McFadden
Mar 4, 2014·International Journal for Parasitology·Christopher D GoodmanGeoffrey I McFadden

❮ Previous
Next ❯

Citations

Nov 24, 2018·F1000Research·Martin Blume, Frank Seeber
Apr 25, 2020·Cell Chemical Biology·Dora PosfaiEmily R Derbyshire
Apr 10, 2021·Frontiers in Cellular and Infection Microbiology·Jennifer RöderMichael Hensel
Sep 30, 2021·Molecular Microbiology·Jessica KimmelTobias Spielmann

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
fluorescence microscopy
affinity-blot
PCR
Transfections
Assay

Software Mentioned

Volocity

Related Concepts

Related Feeds

Antimalarial Agents (ASM)

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.

Antimalarial Agents

Antimalarial agents, also known as antimalarials, are designed to prevent or cure malaria. Discover the latest research on antimalarial agents here.