Host-cell cyclophilin is important for the intracellular replication of Leishmania major

Molecular Microbiology
A HoeraufH Bang

Abstract

The antiparasitic effects of cyclosporin A were examined in leishmanial infection by analysing the role of CsA-binding proteins (cyclophilins) in the host-parasite interaction. We hypothesized that the leishmanicidal effects of CsA on Leishmania major infected macrophages might be mediated through a cyclophilin of either the parasite or the host cell. Two cyclophilins (20 and 22 kDa) were purified from L. major parasites and N-terminally sequenced. Although enzyme activity of these cyclophilins was inhibited by CsA, pretreatment of L. major parasites with CsA did not result in reduction of a subsequent macrophage infection, arguing against a role of L. major cyclophilins as infectivity potentiators. However, host-cell cyclophilin A (CypA) was found to be critically involved in the intracellular replication of L. major parasites in murine macrophages. An antisense oligonucleotide to murine CypA was constructed and added to cultures of peritoneal macrophages prior to infection with L. major parasites. This treatment strongly reduced the expression of CypA in macrophages and resulted in the inhibition of the intracellular replication of L. major amastigotes. These data indicate that interaction of amastigotes with host-cell cyclop...Continue Reading

Citations

May 14, 2011·Medical Microbiology and Immunology·Satyanarayana Swamy CheekatlaSita Naik
Nov 2, 2013·Cell Death & Disease·P NigroM C Capogrossi
Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W DengE W Nester
Jul 6, 2010·Cellular and Molecular Life Sciences : CMLS·Andrzej Galat, Jacqueline Bua
Nov 10, 2007·International Journal for Parasitology·Vyacheslav YurchenkoMichael Bukrinsky
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Can M Ünala, Michael Steinert
Jun 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·H HölschermannW Haberbosch
Jan 26, 2021·Applied Microbiology and Biotechnology·Yating LiaoYanhua Zeng

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