Host cell interactome of PA protein of H5N1 influenza A virus in chicken cells

Journal of Proteomics
Qiao WangGuiping Zhao

Abstract

Influenza A virus (IAV) heavily depends on viral-host protein interactions in order to replicate and spread. Identification of host factors that interact with viral proteins plays crucial roles in understanding the mechanism of IAV infection. Here we report the interaction landscape of H5N1 IAV PA protein in chicken cells through the use of affinity purification and mass spectrometry. PA protein was expressed in chicken cells and PA interacting complexes were captured by co-immunoprecipitation and analyzed by mass spectrometry. A total of 134 proteins were identified as PA-host interacting factors. Protein complexes including the minichromosome maintenance complex (MCM), 26S proteasome and the coat protein I (COPI) complex associated with PA in chicken cells, indicating the essential roles of these functional protein complexes during the course of IAV infection. Gene Ontology and pathway enrichment analysis both showed strong enrichment of PA interacting proteins in the category of DNA replication, covering genes such as PCNA, MCM2, MCM3, MCM4, MCM5 and MCM7. This study has uncovered the comprehensive interactome of H5N1 IAV PA protein in its chicken host and helps to establish the foundation for further investigation into the ...Continue Reading

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Citations

Jul 17, 2016·Journal of Proteomics·Qinghe LiGuiping Zhao
Jul 6, 2019·Molecular Biology and Evolution·Xuexue LiuLin Jiang
Feb 2, 2021·Frontiers in Microbiology·Alessandra RomanoFrancesco Gonella

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