Host Cell Prediction of Exosomes Using Morphological Features on Solid Surfaces Analyzed by Machine Learning

The Journal of Physical Chemistry. B
Kazuki ItoToshio Ogino

Abstract

Exosomes are extracellular nanovesicles released from any cells and found in any body fluid. Because exosomes exhibit information of their host cells (secreting cells), their analysis is expected to be a powerful tool for early diagnosis of cancers. To predict the host cells, we extracted multidimensional feature data about size, shape, and deformation of exosomes immobilized on solid surfaces by atomic force microscopy (AFM). The key idea is combination of support vector machine (SVM) learning for individual exosome particles and their interpretation by principal component analysis (PCA). We observed exosomes derived from three different cancer cells on SiO2/Si, 3-aminopropyltriethoxysilane-modified-SiO2/Si, and TiO2 substrates by AFM. Then, 14-dimensional feature vectors were extracted from AFM particle data, and classifiers were trained in 14-dimensional space. The prediction accuracy for host cells of test AFM particles was examined by the cross-validation test. As a result, we obtained prediction of exosome host cells with the best accuracy of 85.2% for two-class SVM learning and 82.6% for three-class one. By PCA of the particle classifiers, we concluded that the main factors for prediction accuracy and its strong dependen...Continue Reading

References

Jul 31, 2002·Lancet·Fabrice AndreLaurence Zitvogel
Oct 16, 2002·Journal of Immunological Methods·Henry G LamparskiJean-Bernard Le Pecq
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Trairak PisitkunMark A Knepper
Jun 15, 2006·Nature Reviews. Molecular Cell Biology·Jean Gruenberg, F Gisou van der Goot
Dec 13, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Hideki NabikaKei Murakoshi
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charlotte AdmyreSusanne Gabrielsson
Oct 13, 2007·Cell Death and Differentiation·M IeroL Rivoltini
Feb 19, 2008·International Journal of Pharmaceutics·Koji NakanoHirofumi Takeuchi
Mar 6, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Kristian Dimitrievski, Bengt Kasemo
Jun 4, 2008·Biological & Pharmaceutical Bulletin·Yuko OgawaRyohei Yanoshita
Jul 1, 2008·Gynecologic Oncology·Douglas D Taylor, Cicek Gercel-Taylor
Jul 25, 2009·Oral Diseases·A MichaelI Alevizos
Oct 6, 2009·The Journal of Biological Chemistry·Isabella ParoliniStefano Fais
Sep 7, 2006·Physical Chemistry Chemical Physics : PCCP·Ryugo TeroTsuneo Urisu
Oct 21, 2009·Journal of Thrombosis and Haemostasis : JTH·Y YuanaS Osanto
Dec 9, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Kristian Dimitrievski
Jan 29, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Kristian Dimitrievski
Mar 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·D Michiel PegtelJaap M Middeldorp
Apr 1, 2010·The Journal of Biological Chemistry·Nobuyoshi KosakaTakahiro Ochiya
Aug 13, 2010·Kidney International·Giovanni CamussiLuigi Biancone
Oct 1, 2010·Journal of Thrombosis and Haemostasis : JTH·E van der PolR Nieuwland
Jan 18, 2011·Journal of Translational Medicine·Cecilia LässerHadi Valadi
Jun 7, 2011·Colloids and Surfaces. B, Biointerfaces·Viktoriya SokolovaBernd Giebel
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Katrin J SvenssonMattias Belting
Aug 24, 2011·Journal of Proteome Research·Bianca S BatistaLara K Mahal
Oct 7, 2011·Journal of Virology·David G Meckes, Nancy Raab-Traub
Oct 25, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Shivani SharmaJames K Gimzewski
Jul 4, 2012·Advanced Drug Delivery Reviews·Jasper G van den BoornGunther Hartmann
Aug 18, 2012·Immunology Letters·Annamaria MartonKrisztina Buzas
Feb 20, 2013·The Journal of Cell Biology·Graça Raposo, Willem Stoorvogel
Mar 31, 2015·Analytical and Bioanalytical Chemistry·Vasiliy S ChernyshevMikhail Skliar

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biomedical and Environmental Sciences : BES
Jin-Feng WangXiao-Ying Zheng
IEEE Transactions on Image Processing : a Publication of the IEEE Signal Processing Society
Aditya VailayaAnil K Jain
© 2022 Meta ULC. All rights reserved