Host Cell Vimentin Restrains Toxoplasma gondii Invasion and Phosphorylation of Vimentin is Partially Regulated by Interaction with Tg ROP18

International Journal of Biological Sciences
Cheng HeHongjuan Peng

Abstract

The obligate intracellular parasite, Toxoplasma gondii, manipulates the cytoskeleton of its host cells to facilitate infection. A significant rearrangement of host cell vimentin around Toxoplasma parasitophorous vacuoles is observed during the course of infection. ROP18 (TgROP18) is a serine-threonine kinase secreted by T. gondii rhoptry and a major virulence factor; however, the mechanisms by which this kinase modulates host factors remain poorly understood. Different and dynamic patterns of vimentin solubility, phosphorylation, and expression levels were observed in host cells infected with T. gondii strain RH and RH Δrop18 strains, suggesting that TgROP18 contributes to the regulation of these dynamic patterns. Additionally, host cell vimentin was demonstrated to interact with and be phosphorylated by TgROP18. A significant increase in T. gondii infection rate was observed in vimentin knockout human brain microvessel endothelial cells (HBMEC), while vimentin knockout or knock down in host cells had no impact on parasite proliferation and egress. These results indicate that host cell vimentin can inhibit T. gondii invasion. Interestingly, western blotting of different mouse tissues indicated that the lowest vimentin expressio...Continue Reading

Citations

Jul 8, 2020·International Journal of Molecular Sciences·Irene RamosDolores Pérez-Sala
Jun 15, 2021·Frontiers in Cell and Developmental Biology·Lijie YaoHongjuan Peng
Jan 28, 2022·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Min ChenHongjuan Peng

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Methods Mentioned

BETA
GTPases
PCR
fluorescence resonance
co-immunoprecipitation
FRET
Co-IP
fluorescence microscopy
Reverse Transcription PCR

Software Mentioned

Image J

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