PMID: 9420232Jan 7, 1998Paper

Host cis-mediated extinction of a retrovirus permissive for expression in embryonal stem cells during differentiation

Journal of Virology
C LakerC Stocking

Abstract

The use of retroviral vectors for gene transfer into animals has been severely hampered by the lack of provirus transcription in the early embryo and embryonic stem (ES) cells. This primary block in provirus expression is maintained in differentiated cells by a cis-acting mechanism that is not well characterized. Retroviral vectors based on the murine embryonal stem cell virus (MESV), which overcome the transcriptional block in ES cells, were constructed to investigate this secondary mechanism. These vectors transferred G418 resistance to ES cells with the same efficiency as to fibroblasts, but overall transcript levels were greatly reduced. A mosaic but stable expression pattern was observed when single cells from G418-resistant clones were replated in G418 or assayed for expression of LacZ or interleukin-3. The expression levels in independent clones were variable and correlated inversely with methylation. However, a second, more pronounced, block to transcription was found upon differentiation induction. Differentiation of the infected ES cells to cells permissive for retroviral expression resulted in repression and complete extinction of provirus expression. Extinction was not accompanied by increased levels of methylation....Continue Reading

References

Oct 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R JaenischB Croker
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R Jaenisch
Dec 1, 1992·Current Opinion in Genetics & Development·S Henikoff
Jan 1, 1991·Journal of Virology·E AkgünM Grez
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T D PalmerA D Miller
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·R ScharfmannI M Verma
Mar 1, 1991·Molecular and Cellular Biology·R PetersenE Barklis
May 1, 1990·The Journal of Experimental Medicine·G Keller, R Snodgrass
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M GrezW Ostertag
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C StockingW Ostertag
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T FranzW Ostertag
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F HilbergM Grez
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·I Greiser-WilkeJ F Conscience
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·K HarbersR Jaenisch
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C L StewartR Jaenisch
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G RobertsonE Whitelaw
Jan 1, 1994·Annual Review of Biochemistry·S M ParanjapeJ T Kadonaga
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·P M Challita, D B Kohn

❮ Previous
Next ❯

Citations

Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·P B RobbinsD B Kohn
Feb 16, 2000·Gene Therapy·L ZentilinM Giacca
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Alexander PfeiferInder M Verma
May 30, 2015·The Journal of Biological Chemistry·Mi-Sun LimChang-Hwan Park
Dec 7, 2007·Biotechnology & Genetic Engineering Reviews·Xiaofeng Xia, Su-Chun Zhang
Nov 25, 2014·Stem Cells and Development·Pallavi Ananda Kadengodlu, V Ravishankar Rai
Oct 16, 1999·Science·A P Wolffe, M A Matzke
Sep 26, 2000·Molecular and Cellular Biology·S R CherryR Jaenisch
Aug 9, 2002·Molecular and Cellular Biology·Nuray AkyüzLisa Wiesmüller
Aug 19, 2008·Protein Science : a Publication of the Protein Society·Patrick J MillerAndrew D Hollenbach
Sep 26, 2006·Nucleic Acids Research·Philipp KochOliver Brüstle
Dec 23, 2017·International Journal of Molecular Sciences·Jing ZhouPatricia Murray
Jan 25, 2020·Developmental Cell·Jan Tomasz Czermiński, Jeanne Bentley Lawrence
Dec 29, 2020·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Maik HintzeThomas Franz
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·D A HeimC E Dunbar

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.