Host defence during Klebsiella pneumonia relies on haematopoietic-expressed Toll-like receptors 4 and 2

The European Respiratory Journal
C W WielandT van der Poll

Abstract

In this study, the relative roles of Toll-like receptor (TLR)2 and TLR4 were investigated independently and together. Moreover, we studied the role of haematopoietic compartment in anti-Klebsiella host defence. We infected TLR2 and TLR4 single-, and TLR2×4 double knockout (KO) animals with different doses of Klebsiella pneumoniae. In addition, bone marrow chimeric mice were created and infected. TLR4 played a more prominent role in antibacterial defence than TLR2, considering that only TLR4 KO mice demonstrated enhanced bacterial growth in lungs and spleen 24 h after infection with 3×10³ colony-forming units of Klebsiella compared with wild-type (WT) mice. In late-stage infection or after exposure to a higher infectious dose, bacterial counts in lungs of TLR2 KO animals were elevated compared with WT mice and TLR2×4 KO animals were more susceptible to infection than TLR4 KO mice. TLR signalling in cells of haematopoietic origin is of primary importance in host defence against K. pneumoniae. These data suggest that: 1) TLR4 drives the antibacterial host response after induction of pneumonia with relatively low Klebsiella doses; 2) TLR2 becomes involved at a later phase of the infection and/or upon exposure to higher bacterial bu...Continue Reading

Citations

Aug 21, 2012·The Journal of Infectious Diseases·Miriam H P van LieshoutTom van der Poll
Mar 23, 2011·Molecular Medicine·Arie J HoogendijkCatharina W Wieland
Aug 15, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Miriam H P van LieshoutCornelis van't Veer
Apr 12, 2016·Frontiers in Cellular and Infection Microbiology·Michael OstDominik Hartl
Mar 5, 2014·Current Eye Research·Jonathan J HuntMichelle C Callegan
Aug 20, 2014·Communicative & Integrative Biology·Vicky Sender, Cordula Stamme
Mar 19, 2013·The American Journal of Pathology·Jan Willem DuitmanC Arnold Spek
Dec 11, 2013·Critical Care : the Official Journal of the Critical Care Forum·Katharina ChalkMichael Sander
Feb 12, 2017·American Journal of Respiratory Cell and Molecular Biology·Adam A AnasTom van der Poll
Oct 4, 2017·European Journal of Immunology·Miriam H P van LieshoutTom van der Poll
Jun 17, 2016·Microbiology and Molecular Biology Reviews : MMBR·Michelle K Paczosa, Joan Mecsas
Mar 21, 2015·The European Respiratory Journal·Ahmed AchouitiTom van der Poll
Jun 22, 2019·FEMS Microbiology Letters·Sergio Rius-RocabertEstanislao Nistal-Villan
Jun 12, 2016·American Journal of Physiology. Lung Cellular and Molecular Physiology·Adam A AnasTom van der Poll
May 14, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Desiree PerleeAlex F de Vos
Jan 26, 2013·Cellular Microbiology·Christian G FrankJosé A Bengoechea
Mar 29, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yuwei ZhangPing Cheng
Nov 20, 2018·FEMS Microbiology Reviews·José A Bengoechea, Joana Sa Pessoa
Oct 1, 2020·MBio·Joana Sá-PessoaJose A Bengoechea

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