Host-glycan metabolism is regulated by a species-conserved two-component system in Streptococcus pneumoniae

PLoS Pathogens
Patrick Rosendahl AndreassenMikkel Girke Jørgensen

Abstract

Pathogens of the Streptococcus genus inhabit many different environmental niches during the course of an infection in a human host and the bacteria must adjust their metabolism according to available nutrients. Despite their lack of the citric-acid cycle, some streptococci proliferate in niches devoid of a readily available carbohydrate source. Instead they rely on carbohydrate scavenging for energy acquisition, which are obtained from the host. Here we discover a two-component system (TCS07) of Streptococcus pneumoniae that responds to glycoconjugated structures on proteins present on the host cells. Using next-generation RNA sequencing we find that the uncharacterized TCS07 regulon encodes proteins important for host-glycan processing and transporters of the released glycans, as well as intracellular carbohydrate catabolizing enzymes. We find that a functional TCS07 allele is required for growth on the glycoconjugated model protein fetuin. Consistently, we see a TCS07-dependent activation of the glycan degradation pathway. Thus, we pinpoint the molecular constituents responsible for sensing host derived glycans and link this to the induction of the proteins necessary for glycan degradation. Furthermore, we connect the TCS07 r...Continue Reading

References

Aug 1, 1996·Molecular Microbiology·L S HåvarsteinD A Morrison
Aug 1, 1997·Veterinary Research Communications·J J StaatsM M Chengappa
Feb 15, 2000·Molecular Microbiology·J P ThroupM K Burnham
Jun 15, 2000·Journal of Bacteriology·M M Samrakandi, F Pasta
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Mar 7, 2001·Journal of Clinical Microbiology·K A HomerD Beighton
Sep 7, 2001·Journal of Bacteriology·J HoskinsJ I Glass
Nov 18, 2004·Journal of Bacteriology·Andrew T UlijaszBernard Weisblum
May 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Alistair J StandishJames C Paton
Nov 8, 2005·Journal of Clinical Microbiology·Jørn A AasFloyd E Dewhirst
Dec 13, 2005·Current Opinion in Microbiology·Sven Hammerschmidt
Jan 27, 2006·Microbiology·Tomas G KloostermanOscar P Kuipers
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Matthew A WrightDaniel Segrè
Sep 21, 2007·Molecular Microbiology·Ramkumar Iyer, Andrew Camilli
Mar 15, 2008·Nature Reviews. Microbiology·Aras KadiogluPeter W Andrew
Jun 13, 2008·Nature Protocols·Thomas D Schmittgen, Kenneth J Livak
Jan 14, 2009·Infection and Immunity·Carolyn MarionSamantha J King
Apr 28, 2009·FEMS Microbiology Reviews·Ola Johnsborg, Leiv Sigve Håvarstein
Jul 29, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Claudia M Brandt, Barbara Spellerberg
Feb 20, 2010·PLoS Computational Biology·Sara BallouzMark M Tanaka
Nov 1, 2011·PloS One·Sandra M CarvalhoAna Rute Neves
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Mar 20, 2014·Bioinformatics·Torsten Seemann

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Citations

Jul 19, 2021·Trends in Microbiology·Sohyoung LeeJeongmin Song
Sep 22, 2021·MBio·Dhriti SinhaNicholas R De Lay

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Datasets Mentioned

BETA
GSE132733

Methods Mentioned

BETA
RNA-seq
glycosylation
fluorescence microscopy
PCR

Software Mentioned

Bowtie2
Scoary
FastTree
lme4
Circlize
iTOL
Bioconductor package EdgeR
R
Prokka
ME

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