Aug 23, 2005

Host immune status influences the development of attaching and effacing lesions in weaned pigs

Infection and Immunity
Francis GirardJ M Fairbrother

Abstract

Attaching and effacing Escherichia coli (AEEC) has been associated with naturally occurring attaching and effacing (A/E) lesions in weaned pigs, and although A/E lesions have been experimentally reproduced in newborn piglets, such lesions have been much more difficult to induce in older conventional pigs. Hence, the aim of this study was to examine the effect of oral administration of dexamethasone on the development of A/E lesions in weaned pigs challenged with a porcine enteropathogenic E. coli (PEPEC) strain and to investigate the involvement of local intestinal cytokine response. Dexamethasone, given orally at a dosage of 3 mg kg of body weight(-1), significantly enhanced both the colonization of the challenge strain and the prevalence of foci of intimately adherent bacteria, resulting in extensive A/E lesions in the ileum, cecum, and colon of challenged pigs. We also confirmed the expression of both intimin and Tir by PEPEC strain ECL1001 in A/E lesions in vivo, which is, to our knowledge, the first report of the involvement of the latter proteins in any AEEC infections in vivo. Moreover, semiquantitative reverse transcription-PCR demonstrated that interleukin 1beta (IL-1beta), IL-6, IL-8, and, to a lesser extent, IL-12p40...Continue Reading

Mentioned in this Paper

Immunoblotting, Reverse
Alkalescens-Dispar Group
Tir protein, E coli
Hormone Receptors, Cell Surface
Anti-Inflammatory Agents
Ileum
Weighing Patient
Escherichia Coli Infections
Swine Diseases
Entire Ileum

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