Host MicroRNA hsa-miR-494-3p Promotes EV71 Replication by Directly Targeting PTEN

Frontiers in Cellular and Infection Microbiology
Qing ZhaoBo Wang

Abstract

Many cellular processes are driven by spatially and temporally regulated microRNAs (miRNAs)-dependent signaling events. Substantial evidence collected over the years indicates that miRNAs are pivotal regulators that contribute to the initiation and development of EV71-related disorders. Importantly, so far, no clinical trial has been undertaken to address the effect of miRNAs on EV71-related diseases. In this study, we show that EV71 infection results in up-regulation of hsa-miR-494-3p levels, and that EV71-induced hsa-miR-494-3p impacts PI3K/Akt signaling pathway by targeting PTEN. However, very little is known about the relationship between hsa-miR-494-3p and EV71 infection. The overall goal of the study is to get a better insight into whether or not hsa-miR-494-3p is involved in the EV71 infection. We found that the EV71 infection induces cellular apoptosis, and that this process can be counteracted by the over-expression of hsa-miR-494-3p mimics. We also present evidence that cell lines deficient in hsa-miR-494-3p are more sensitive to EV71-induced cell death than the corresponding control cells. Collectively, these findings confirm and extend the pervious observation suggesting that disturbances in miRNAs expression can in...Continue Reading

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Citations

Sep 23, 2020·Journal of Clinical Laboratory Analysis·Caixia LiJin-Hui Li
May 14, 2020·Cellular & Molecular Immunology·Dong LiGuangcai Duan
Jun 26, 2020·Frontiers in Microbiology·Yalan LaiXiaoyue Chen
Apr 4, 2021·International Journal of Molecular Sciences·Peiyu ZhuYuefei Jin

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Methods Mentioned

BETA
ubiquitination
transfection
PCR
Protein Assay
electrophoresis

Software Mentioned

VassarStats
qRCR
cellSens
TargetScan

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