Host Mucin Is Exploited by Pseudomonas aeruginosa To Provide Monosaccharides Required for a Successful Infection.

MBio
C. L. HoffmanAlejandro Aballay

Abstract

One of the primary functions of the mucosal barrier, found lining epithelial cells, is to serve as a first-line of defense against microbial pathogens. The major structural components of mucus are heavily glycosylated proteins called mucins. Mucins are key components of the innate immune system as they aid in the clearance of pathogens and can decrease pathogen virulence. It has also been recently reported that individual mucins and derived glycans can attenuate the virulence of the human pathogen Pseudomonas aeruginosa Here, we show data indicating that mucins not only play a role in host defense but that they can also be subverted by P. aeruginosa to cause disease. We found that the mucin MUL-1 and mucin-derived monosaccharides N-acetyl-galactosamine and N-acetylglucosamine are required for P. aeruginosa killing of Caenorhabditis elegans We also found that the defective adhesion of P. aeruginosa to human lung alveolar epithelial cells, deficient in the mucin MUC1, can be reversed by the addition of individual monosaccharides. The monosaccharides identified in this study are found in a wide range of organisms where they act as host factors required for bacterial pathogenesis. While mucins in C. elegans lack sialic acid caps, w...Continue Reading

References

Nov 1, 1990·Experimental Parasitology·A BacicB M Zuckerman
Jan 1, 1987·Antibiotics and Chemotherapy·R U Sorensen, J D Klinger
Oct 1, 1996·American Journal of Respiratory and Critical Care Medicine·S LoryP Bergman
Jan 20, 1999·Proceedings of the National Academy of Sciences of the United States of America·M W TanF M Ausubel
Feb 25, 1999·American Journal of Respiratory Cell and Molecular Biology·J T BergerM C Rose
Nov 5, 1999·Methods in Enzymology·G A O'TooleR Kolter
Mar 16, 2000·Molecular and Cellular Biochemistry·D B VimalN K Ganguly
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Erik P LillehojK Chul Kim
Aug 15, 2002·Current Biology : CB·Gustavo V MalloJonathan J Ewbank
Oct 4, 2002·The Journal of Biological Chemistry·John F CipolloCarlos B Hirschberg
Jul 15, 2003·Molecular Biology of the Cell·Lisa TimmonsAndrew Z Fire
Nov 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Huimin RanGee W Lau
Nov 17, 2004·The Journal of Biological Chemistry·David W ZaasSoman N Abraham
Jan 13, 2005·Methods in Enzymology·Juan Wang, Maureen M Barr
Mar 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wenju LuK Chul Kim
Apr 4, 2006·Annals of Medicine·Duncan F Rogers, Peter J Barnes
Jul 25, 2006·The Journal of Clinical Investigation·Grace SoongAlice Prince
Oct 4, 2008·Microbiology·Niels P BoksHenk J Busscher
Dec 17, 2008·Mucosal Immunology·S K LindenM A McGuckin
Dec 18, 2008·PloS One·Sara K LindénMichael A McGuckin
Nov 26, 2009·Glycoconjugate Journal·Phillip ParkerStuart Smith
Dec 29, 2009·Frontiers in Bioscience (Elite Edition)·Kosuke KatoKwang Chul Kim
Feb 5, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Frederick A PartridgeJonathan Hodgkin
Dec 24, 2010·Genome Research·W Clay SpencerDavid M Miller
Mar 7, 2012·Microbiology and Molecular Biology Reviews : MMBR·Pol Nadal JimenezWim J Quax

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Citations

Jul 7, 2020·FEMS Microbiology Reviews·Ashley Valle Arevalo, Clarissa J Nobile
Mar 3, 2021·Lab Animal·Cassandra BackesFilipe Cabreiro
Jul 3, 2021·Biomedicines·Oliver W Meldrum, Sanjay H Chotirmall
Jul 19, 2021·Trends in Microbiology·Sohyoung LeeJeongmin Song
Jun 29, 2021·Virulence·Charles D MorinGee W Lau

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Datasets Mentioned

BETA
SM022

Methods Mentioned

BETA
glycosylation
transgenic
transfection

Software Mentioned

Prism
GraphPad Prism
GlycoPred
GraphPad

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