PMID: 9524444Apr 1, 1997Paper

Host-pathogen interactions in bacterial endocarditis: streptococcal virulence in the host

Advances in Dental Research
M C HerzbergL Tao

Abstract

To identify streptococcal genes that are expressed during experimental endocarditis, we developed a promoter-less dual reporter gene-fusion (amy, cat) plasmid, pAK36. Chromosomal DNA from S. gordonii V288 was digested with Sau3A1. The resulting fragments were ligated into pAK36. Following transformation into S. gordonii, the library of random gene fusion clones was inoculated into a rabbit to induce experimental endocarditis. Chloramphenicol treatment effected positive selection. Upon euthanization of the rabbits, the valvular vegetations were excised in a sterile field. Surviving clones were isolated and screened in vitro for chloramphenicol sensitivity and negative amylase activity. From the 48 randomly picked, double-negative clones, DNA was isolated and analyzed by Southern hybridization with labeled pAK36 probe. Different insertion patterns were identified, suggesting that no fewer than 13 S. gordonii genes were induced. Therefore, S. gordonii genes are induced during experimental endocarditis, which may contribute to virulence.

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Citations

Nov 30, 2000·Current Infectious Disease Reports·J K McCormickP M Schlievert
Jul 10, 2001·Oral Microbiology and Immunology·Y Liu, H M Fletcher
May 14, 2009·DNA and Cell Biology·Jens KrethFengxia Qi
Aug 6, 2008·Infection and Immunity·Biswendu ChaudhuriFrank A Scannapieco
Sep 6, 2006·The Journal of Medical Investigation : JMI·Hiro-O Ito
Oct 8, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Howard K Kuramitsu
Sep 28, 2006·Journal of Oral Rehabilitation·M SatoH Miyazaki
Mar 1, 2008·Proteomics. Clinical Applications·Jason N ColeMark J Walker
Jul 24, 2003·International Dental Journal·Michael Martin
Mar 5, 2004·Oral Diseases·K OkudaK Ishihara
Oct 6, 2017·Annals of Clinical Microbiology and Antimicrobials·Ziv DadonGabriel Munter
Nov 2, 2019·Scientific Reports·Nathan D PalankerBingyan Wang

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