Host plant genome overcomes the lack of a bacterial gene for symbiotic nitrogen fixation.

Nature
Tsuneo HakoyamaNorio Suganuma

Abstract

Homocitrate is a component of the iron-molybdenum cofactor in nitrogenase, where nitrogen fixation occurs. NifV, which encodes homocitrate synthase (HCS), has been identified from various diazotrophs but is not present in most rhizobial species that perform efficient nitrogen fixation only in symbiotic association with legumes. Here we show that the FEN1 gene of a model legume, Lotus japonicus, overcomes the lack of NifV in rhizobia for symbiotic nitrogen fixation. A Fix(-) (non-fixing) plant mutant, fen1, forms morphologically normal but ineffective nodules. The causal gene, FEN1, was shown to encode HCS by its ability to complement a HCS-defective mutant of Saccharomyces cerevisiae. Homocitrate was present abundantly in wild-type nodules but was absent from ineffective fen1 nodules. Inoculation with Mesorhizobium loti carrying FEN1 or Azotobacter vinelandii NifV rescued the defect in nitrogen-fixing activity of the fen1 nodules. Exogenous supply of homocitrate also recovered the nitrogen-fixing activity of the fen1 nodules through de novo nitrogenase synthesis in the rhizobial bacteroids. These results indicate that homocitrate derived from the host plant cells is essential for the efficient and continuing synthesis of the ni...Continue Reading

References

Feb 1, 1995·The Plant Cell·K Jacobsen-LyonE S Dennis
Aug 1, 1997·Molecular Plant-microbe Interactions : MPMI·K TakaneH Kouchi
Sep 19, 1997·Journal of Bacteriology·L ZhengD R Dean
Feb 15, 2002·Molecular Plant-microbe Interactions : MPMI·Masayoshi KawaguchiShoichiro Akao
Apr 10, 2003·Molecular Genetics and Genomics : MGG·N SuganumaM Kawaguchi
Nov 24, 2004·Molecular Plant-microbe Interactions : MPMI·Norio SuganumaHiroshi Kouchi
Sep 10, 2005·Photosynthesis Research·Eric Giraud, Darrell Fleischman
Jan 1, 1981·Applied and Environmental Microbiology·D GauthierY Dommergues
Feb 1, 1983·Applied and Environmental Microbiology·B L DreyfusY R Dommergues
Jul 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C W RonsonJ G Robertson
Apr 6, 2007·BMC Evolutionary Biology·Christoph KneipUwe-G Maier
Jun 2, 2007·Science·Eric GiraudMichael Sadowsky
May 31, 2008·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Shusei SatoSatoshi Tabata
Jul 15, 2009·Proceedings of the National Academy of Sciences of the United States of America·J PrellP S Poole

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Citations

Jul 28, 2010·Plant & Cell Physiology·Hiroshi KouchiMasayoshi Kawaguchi
May 3, 2013·Molecular Plant-microbe Interactions : MPMI·Jiejun XiRujin Chen
Oct 15, 2011·Molecular Plant-microbe Interactions : MPMI·Attila KeresztEva Kondorosi
Sep 13, 2013·Annual Review of Microbiology·Eva KondorosiAttila Kereszt
Mar 5, 2013·Annual Review of Plant Biology·Michael Udvardi, Philip S Poole
Aug 16, 2011·Annual Review of Genetics·Giles E D OldroydJ Allan Downie
Jan 8, 2015·Frontiers in Plant Science·Victoria C ClarkePenelope M C Smith
Jul 20, 2016·Microbes and Environments·Takashi OkuboKiwamu Minamisawa
Nov 1, 2016·Genome Announcements·Yoshikazu ShimodaMakoto Hayashi
Jan 31, 2018·Nature Reviews. Microbiology·Philip PooleJason Terpolilli
Feb 20, 2018·Nature Reviews. Microbiology·Brittany J BelinDianne K Newman
Dec 16, 2017·The Plant Journal : for Cell and Molecular Biology·Sheila McCormick
Aug 3, 2018·Molecular Plant-microbe Interactions : MPMI·Maged M SaadXavier Perret
May 15, 2013·Molecular Systems Biology·Nicola SegataCurtis Huttenhower
Mar 8, 2011·Cellular and Molecular Life Sciences : CMLS·Keisuke Yokota, Makoto Hayashi
Jan 5, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yoshikazu ShimodaMakoto Hayashi
May 23, 2019·Physiologia Plantarum·Akito ImaiNorio Suganuma
Mar 24, 2020·Chembiochem : a European Journal of Chemical Biology·Maame A Addo, Patricia C Dos Santos
Jul 31, 2020·Microbes and Environments·Teerana GreetatornNeung Teaumroong
Dec 5, 2019·Microbial Biotechnology·Kristina Lindström, Seyed Abdollah Mousavi
Dec 18, 2019·Nature Microbiology·Min-Hyung RyuChristopher A Voigt
Feb 7, 2018·Frontiers in Plant Science·Teodoro Coba de la PeñaM Mercedes Lucas
Feb 3, 2021·Journal of Bacteriology·Raphael LedermannPhilip S Poole

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