Host response during Yersinia pestis infection of human bronchial epithelial cells involves negative regulation of autophagy and suggests a modulation of survival-related and cellular growth pathways

Frontiers in Microbiology
Farhang AlemRamin M Hakami

Abstract

Yersinia pestis (Yp) causes the re-emerging disease plague, and is classified by the CDC and NIAID as a highest priority (Category A) pathogen. Currently, there is no approved human vaccine available and advances in early diagnostics and effective therapeutics are urgently needed. A deep understanding of the mechanisms of host response to Yp infection can significantly advance these three areas. We employed the Reverse Phase Protein Microarray (RPMA) technology to reveal the dynamic states of either protein level changes or phosphorylation changes associated with kinase-driven signaling pathways during host cell response to Yp infection. RPMA allowed quantitative profiling of changes in the intracellular communication network of human lung epithelial cells at different times post infection and in response to different treatment conditions, which included infection with the virulent Yp strain CO92, infection with a derivative avirulent strain CO92 (Pgm-, Pst-), treatment with heat inactivated CO92, and treatment with LPS. Responses to a total of 111 validated antibodies were profiled, leading to discovery of 12 novel protein hits. The RPMA analysis also identified several protein hits previously reported in the context of Yp inf...Continue Reading

References

Apr 3, 1992·Cell·G I EvanD C Hancock
Jul 28, 1995·Biochimica Et Biophysica Acta·G Packham, J L Cleveland
May 23, 1998·The Journal of Biological Chemistry·R U JänickeA G Porter
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Jun 23, 1999·Oncogene·E V Schmidt
Jun 29, 2000·Annual Review of Biochemistry·A J Shaywitz, M E Greenberg
Aug 22, 2000·The Journal of Biological Chemistry·J M DukuzumuremyiK Schesser
Mar 30, 2001·Cell Death and Differentiation·T E AllsoppJ S Kelly
May 19, 2001·European Journal of Biochemistry·S H Liang, M F Clarke
Aug 3, 2001·Nature Reviews. Molecular Cell Biology·B Mayr, M Montminy
Aug 7, 2002·The Journal of Cell Biology·Guy R Cornelis
Aug 13, 2002·Nature Cell Biology·Ken InokiKun-Liang Guan
Oct 3, 2002·Nature Reviews. Molecular Cell Biology·Guy R Cornelis
Apr 25, 2003·International Journal of Pharmaceutics·Ben ForbesAlison B Lansley
Aug 19, 2003·The Journal of Biological Chemistry·Toshiyuki ArakiBenjamin G Neel
Jan 28, 2004·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Aurora O'Brate, Paraskevi Giannakakou
Mar 25, 2004·Journal of Cellular Physiology·Christina Ivins ZitoAnton M Bennett
Jun 24, 2004·Seminars in Cell & Developmental Biology·Julian Downward
Feb 22, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Céline Pujol, James B Bliska
Mar 24, 2005·Journal of Cellular and Molecular Medicine·Gang SongShideng Bao
Apr 26, 2005·Annual Review of Microbiology·Gloria I Viboud, James B Bliska
Jun 28, 2005·Journal of Clinical Immunology·Rajesh Kumar SharmaHarsh Vardhan Batra
Mar 7, 2006·Developmental Cell·Wentian YangBenjamin G Neel
Apr 26, 2006·The Journal of Cell Biology·Sheng-Li CaiCheryl Lyn Walker
Sep 21, 2006·Chemistry & Biology·Ai-Sun TsengMark T Keating

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Citations

Jul 22, 2016·Proteomics. Clinical Applications·Erik RichterFalko Hochgräfe
May 6, 2017·American Journal of Physiology. Lung Cellular and Molecular Physiology·Zhou-Yang LiZhi-Hua Chen
Jul 5, 2017·Experimental and Therapeutic Medicine·You-Ping DengPin Liu
Apr 28, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Adeyemi A Olanrewaju, Ramin M Hakami

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Methods Mentioned

BETA
transfections
Confocal microscopy

Software Mentioned

RPMA

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