May 7, 2015

Host Response to the Lung Microbiome in Chronic Obstructive Pulmonary Disease

American Journal of Respiratory and Critical Care Medicine
Marc A SzeJames C Hogg

Abstract

The relatively sparse but diverse microbiome in human lungs may become less diverse in chronic obstructive pulmonary disease (COPD). This article examines the relationship of this microbiome to emphysematous tissue destruction, number of terminal bronchioles, infiltrating inflammatory cells, and host gene expression. Culture-independent pyrosequencing microbiome analysis was used to examine the V3-V5 regions of bacterial 16S ribosomal DNA in 40 samples of lung from 5 patients with COPD (Global Initiative for Chronic Obstructive Lung Disease [GOLD] stage 4) and 28 samples from 4 donors (controls). A second protocol based on the V1-V3 regions was used to verify the bacterial microbiome results. Within lung tissue samples the microbiome was compared with results of micro-computed tomography, infiltrating inflammatory cells measured by quantitative histology, and host gene expression. Ten operational taxonomic units (OTUs) was found sufficient to discriminate between control and GOLD stage 4 lung tissue, which included known pathogens such as Haemophilus influenzae. We also observed a decline in microbial diversity that was associated with emphysematous destruction, remodeling of the bronchiolar and alveolar tissue, and the infiltr...Continue Reading

  • References25
  • Citations44

References

Mentioned in this Paper

Microorganism
Eosinophil
Immune Response
Pathogenic Aspects
Chronic Airflow Obstruction
T-Lymphocyte
Pathogenesis
Histology Procedure
Analysis Using Pyrosequencing
Haemophilus influenzae

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