Household Microbial Water Quality Testing in a Peruvian Demographic and Health Survey: Evaluation of the Compartment Bag Test forEscherichia coli

The American Journal of Tropical Medicine and Hygiene
Alice WangM D Sobsey

Abstract

AbstractThe Joint Monitoring Program relies on household surveys to classify access to improved water sources instead of measuring microbiological quality. The aim of this research was to pilot a novel test forEscherichia coliquantification of household drinking water in the 2011 Demographic and Health Survey (DHS) in Peru. In the Compartment Bag Test (CBT), a 100-mL water sample is supplemented with chromogenic medium to support the growth ofE. coli, poured into a bag with compartments, and incubated. A color change indicatesE. coligrowth, and the concentration ofE. coli/100 mL is estimated as a most probable number. Triplicate water samples from 704 households were collected; one sample was analyzed in the field using the CBT, another replicate sample using the CBT was analyzed by reference laboratories, and one sample using membrane filtration (MF) was analyzed by reference laboratories. There were no statistically significant differences inE. coliconcentrations between the field and laboratory CBT results, or when compared with MF results. These results suggest that the CBT forE. coliis an effective method to quantify fecal bacteria in household drinking water. The CBT can be incorporated into DHS and other national househo...Continue Reading

References

Jan 1, 1983·Advances in Applied Microbiology·G A McFeters, A K Camper
Mar 1, 1981·American Journal of Public Health·G R BrennimanR L Northrop
Jan 11, 2011·Journal of Applied Microbiology·J BrownM D Sobsey
Nov 1, 2011·Applied Microbiology and Biotechnology·Lanakila McMahanMark D Sobsey
Jun 13, 2012·International Journal of Environmental Research and Public Health·Kyle OndaJamie Bartram
Jul 4, 2012·International Journal of Environmental Research and Public Health·Robert BainStephen Gundry
Feb 26, 2014·Journal of Microbiological Methods·Christine StauberKate Kroell
Apr 5, 2014·Bulletin of the World Health Organization·Ameer ShaheedJoe Brown
May 9, 2014·Tropical Medicine & International Health : TM & IH·Robert BainJamie Bartram
Jul 22, 2014·International Journal of Environmental Research and Public Health·Jonny Crocker, Jamie Bartram

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