How AraC interacts specifically with its target DNAs

Journal of Molecular Biology
P NilandB Müller-Hill

Abstract

Previous work indicates that one subunit of the AraC protein dimer binds to a DNA target araI1, of 17 base-pairs. We systematically substituted every base-pair in a synthetic araI1 target with the three possible alternatives and then tested binding of araI1 and of these 51 DNA targets to AraC by quantitative gel shift analysis in the presence of L-arabinose. We found that every substitution of the underlined bases reduces AraC binding tenfold or more: 5' TAGCATTTTTATCCATA 3'. Substitutions at other bases have little or no effect. In the absence of L-arabinose we observed a sixfold reduction of binding of AraC to araI1. We have designated the 5' AGC sequence the A-box and the 5'TCCATA sequence the B-box. We synthesised DNA targets containing either two A or two B-boxes with the natural araI1-I2 spacing. Wild-type AraC binds both targets in the presence of L-arabinose in a gel shift-experiment. In the absence of L-arabinose, AraC binds only to the double B-box. We then tested various AraC mutant proteins in the same way. S208A and H212A bind to the double B-box but not to the double A-box in the presence or absence of L-arabinose. D256A binds to the double A-box, but not to the double B-box, in the presence of L-arabinose but not...Continue Reading

Citations

Nov 28, 2007·Nucleic Acids Research·Michael E Rodgers, Robert Schleif
Apr 7, 2010·Journal of Bacteriology·Patricia Domínguez-CuevasSilvia Marqués
Sep 25, 2002·Journal of Bacteriology·Susan M Egan
Sep 25, 2002·Journal of Bacteriology·Robin R Hulbert, Ronald K Taylor
Oct 5, 2011·Molecular and Cellular Biology·Jeffrey N McKnightGregory D Bowman
Nov 22, 2015·Molecular Biology and Evolution·Mato LagatorJonathan P Bollback
Dec 3, 2014·Chemistry & Biology·Isabelle T Holder, Jörg S Hartig
Jun 30, 2004·Journal of Molecular Biology·Andrew TimmesRobert Schleif
Sep 12, 2018·Molecular Systems Biology·Yolanda SchaerliAndreas Wagner
Oct 5, 2001·The Journal of Biological Chemistry·R Gurezka, D Langosch
May 8, 2009·Proteins·Michael E Rodgers, Robert Schleif
Apr 3, 2016·Molecular Microbiology·Araceli E SantiagoJames P Nataro
May 3, 2019·Applied Microbiology and Biotechnology·Varsha P Chhiba-GovindjeeDean Brady
Aug 28, 1999·Journal of Bacteriology·P M Bhende, S M Egan
Nov 21, 2019·Cell Reports·Drake A DonovanJeffrey N McKnight
May 9, 1998·Journal of Bacteriology·J Bassias, R Brückner
Apr 2, 2021·Nature Communications·Christopher R HorneRenwick C J Dobson
Sep 14, 2001·Biochemical and Biophysical Research Communications·R Ruiz, J L Ramos
Dec 31, 1997·Microbiology and Molecular Biology Reviews : MMBR·M T GallegosJ L Ramos

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