PMID: 15373961Sep 18, 2004Paper

How are genes measured? Examples from studies on iron metabolism in pregnancy

The Proceedings of the Nutrition Society
Henriette S Andersen, Harry J McArdle

Abstract

As the 21st century moves forward, it is becoming more and more apparent that the genetic makeup of any individual strongly influences the way they metabolise nutrients. It is very important, therefore, to understand the techniques and technologies used to assess the contribution genes make to the physiology of an individual. Clearly, it is not possible to provide a comprehensive overview, but in the present review an attempt will be made to show, using examples from the authors' research, how these methods have contributed to this understanding. Studies are being undertaken into Fe transport across the placenta, from the mother to the fetus, and the consequences of maternal anaemia on pregnancy outcome. Levels of gene transcript and protein have been measured using Northern and Western blotting respectively. During the course of this work a new protein has been identified using the available human genome database. Following this 'in silico' or 'cyber biology', techniques such as real-time RT-PCR and RNA interference have been used to examine expression of this gene and its protein. The methods used, briefly how they work and some of their limitations will be explained. The objective of the present review is primarily to give a...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Oct 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·S D Clarke, S Abraham
Jun 1, 1990·Journal of Clinical Pathology·D P JacksonP Quirke
Mar 1, 1988·Journal of Cellular Physiology·H J McArdle, J Tysoe
Sep 1, 1985·Journal of Cellular Physiology·H J McArdleE H Morgan
Dec 1, 1970·The Journal of Clinical Investigation·H P RoeserG E Cartwright
Sep 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P S Thomas
Nov 1, 1982·Journal of Reproduction and Fertility·H J McArdle, E H Morgan
Sep 1, 1993·British Journal of Obstetrics and Gynaecology·A A FryerJ E Bell
Nov 8, 1996·Science·M R Emmert-BuckL A Liotta
Aug 5, 1998·Current Opinion in Genetics & Development·J H Nadeau, P J Dunn
Dec 10, 1998·Nucleic Acids Research·D A BensonD L Wheeler
May 14, 1999·Trends in Genetics : TIG·E G Jennings, R A Young
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·Z L HarrisJ D Gitlin
Nov 26, 1999·Nucleic Acids Research·M J SingerR M Wydro
Dec 28, 1999·BioFactors·C Carlberg
Nov 22, 2000·Journal of Biochemical and Biophysical Methods·T D Schmittgen, B A Zakrajsek
Feb 13, 2001·Trends in Genetics : TIG·H Vaucheret, M Fagard
Mar 17, 2001·Nature Reviews. Genetics·S M HammondG J Hannon
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·N J CaplenR A Morgan
Dec 12, 2001·Molecular and Cellular Probes·S SelveyL R Griffiths
Jan 17, 2002·The Journal of Surgical Research·Brian S ConklinChangyi Chen
Feb 8, 2002·American Journal of Physiology. Cell Physiology·Ruth DanzeisenHarry J McArdle
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·C T WittwerK S Elenitoba-Johnson
Mar 21, 2002·The Biochemical Journal·Malgorzata KisielowYoshikuni Nagamine
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
May 10, 2002·Nucleic Acids Research·Olivier Donzé, Didier Picard
Jun 11, 2002·Methods : a Companion to Methods in Enzymology·Sayda M ElbashirThomas Tuschl
Jul 12, 2002·Nature·Gregory J Hannon

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Oct 5, 2013·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Yusuf Erşahin
Mar 9, 2006·The American Journal of Clinical Nutrition·Mari S GolubBetsy Lozoff

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