How cell culture conditions affect the microstructure and nanomechanical properties of extracellular matrix formed by immortalized human mesenchymal stem cells: An experimental and modelling study

Materials Science & Engineering. C, Materials for Biological Applications
Pengfei DuanJinju Chen

Abstract

This paper presents an investigation of how different culture media (i.e. basal and osteogenic media) affect the nanomechanical properties and microstructure of the mineralized matrix produced by the human mesenchymal stem cell line Y201, from both an experimental and theoretical approach. A bone nodule (i.e. mineralized matrix) cultured from basal medium shows a more anisotropic microstructure compared to its counterpart cultured from an osteogenic medium. As confirmed by finite element simulations, this anisotropic microstructure explains the bimodal distribution of the corresponding mechanical properties very well. The overall nanomechanical response of the bone nodule from the osteogenic medium is poorer compared to its counterpart from the basal medium. The bone nodules, from both basal and osteogenic media, have shown reverse aging effects in terms of mechanical properties. These are possibly due to the fact that cell proliferation outcompetes the mineralization process.

Citations

Sep 29, 2019·Clinical Hemorheology and Microcirculation·Wing Tai TungAndreas Lendlein
Dec 19, 2019·International Journal of Molecular Sciences·M Piñeiro-RamilS M Díaz-Prado
Dec 14, 2018·Micromachines·Long Qian, Hongwei Zhao
Feb 14, 2021·Materials Science & Engineering. C, Materials for Biological Applications·L VaianiA Boccaccio
Jun 5, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Jun Kit WangChor Yong Tay
Dec 5, 2018·Biomacromolecules·Damla KeskinPatrick van Rijn
Jan 5, 2022·Langmuir : the ACS Journal of Surfaces and Colloids·Ma LuoJinju Chen

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