How do the full-generation poly(amido)amine (PAMAM) dendrimers activate blood platelets? Activation of circulating platelets and formation of "fibrinogen aggregates" in the presence of polycations

International Journal of Pharmaceutics
Cezary WatalaMagdalena Labieniec-Watala

Abstract

Direct use of poly(amido)amine (PAMAM) dendrimers as drugs may be limited, due to uncertain (cyto)toxicity. Peripheral blood components, which constitute the first line of a contact with administered pharmaceuticals, may become vastly affected by PAMAM dendrimers. The aim of this study was to explore how PAMAMs' polycationicity might affect blood platelet activation and reactivity, and thus trigger various haemostatic events. We monitored blood platelet reactivity in rats with experimental diabetes upon a long-term administration of the unmodified PAMAM dendrimers. In parallel, the effects on blood flow in a systemic circulation was recorded intravitally in mice administered with PAMAM G2, G3 or G4. Compounding was the in vitro approach to monitor the impact of PAMAM dendrimers on blood platelet activation and reactivity and on selected haemostatic and protein conformation parameters. We demonstrated the activating effects of polycations on blood platelets. Some diversity of the revealed outcomes considerably depended on the used approach and the particular technique employed to monitor blood platelet function. We discovered undesirable impact of plain PAMAM dendrimers on primary haemostasis and their prothrombotic influence. W...Continue Reading

References

Feb 1, 1980·Journal of Pharmacological Methods·D C Cardinal, R J Flower
Jan 12, 2002·Bioelectrochemistry·B Klajnert, M Bryszewska
Feb 14, 2006·Thrombosis Research·Magdalena BonclerCezary Watala
Feb 16, 2006·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Cezary WatalaJózef Drzewoski
May 6, 2006·Biochemical and Biophysical Research Communications·B KlajnertM Bryszewska
Dec 2, 2006·Thrombosis and Haemostasis·Orsolya TóthWolfgang Siess
Sep 2, 2008·International Journal of Pharmaceutics·Magdalena LabieniecCezary Watala
Nov 6, 2008·Toxicology and Applied Pharmacology·N A Monteiro-RiviereL W Zhang
Jan 19, 2010·Journal of Materials Science. Materials in Medicine·Mohammad Shafiee AlavidjehHassan Namazi
Jun 22, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·R M ZuckerW K Boyes
Oct 27, 2011·Molecular Pharmaceutics·Marina A DobrovolskaiaScott E McNeil
Apr 14, 2012·Molecular Pharmaceutics·Clinton F JonesDavid W Grainger
Jul 8, 2014·International Journal of Pharmaceutics·Magdalena Markowicz-PiaseckaJoanna Sikora

❮ Previous
Next ❯

Citations

Sep 3, 2019·Clinical Hemorheology and Microcirculation·Guanhua LiJunmeng Zheng
Apr 2, 2020·Materials Science & Engineering. C, Materials for Biological Applications·Roza AisinaElizaveta Ivanova
Jul 11, 2021·The Journal of Pharmacy and Pharmacology·Tahereh Zadeh Mehrizi, Sedigheh Amini Kafiabad

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.