How does tmRNA move through the ribosome?

FEBS Letters
Pavel V IvanovKnud H Nierhaus

Abstract

To test the structure of tmRNA in solution, cross-linking experiments were performed which showed two sets of cross-links in two different domains of tmRNA. Site-directed mutagenesis was used to search for tmRNA nucleotide bases that might form a functional analogue of a codon-anticodon duplex to be recognized by the ribosomal A-site. We demonstrate that nucleotide residues U85 and A86 from tmRNA are significant for tmRNA function and propose that they are involved in formation of a tmRNA element playing a central role in A-site recognition. These data are discussed in the frame of a hypothetical model that suggests a general scheme for the interaction of tmRNA with the ribosome and explains how it moves through the ribosome.

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Citations

Jun 17, 2005·BMC Molecular Biology·Jody BurksJacek Wower
Jul 2, 2008·BMC Biology·Mickey R MillerAllen R Buskirk
Apr 3, 2007·RNA·Natalia IvanovaMåns Ehrenberg
Oct 14, 2010·The EMBO Journal·Jie FuJoachim Frank
Jan 15, 2010·IUBMB Life·Olga V ShpanchenkoOlga A Dontsova
May 6, 2003·Annual Review of Microbiology·Jeffrey H Withey, David I Friedman
Feb 2, 1999·Journal of Molecular Biology·M S VanLoockS C Harvey
Feb 17, 2005·The Journal of Biological Chemistry·Olga V ShpanchenkoOlga A Dontsova
Aug 20, 2014·RNA·Ehsan Ranaei-SiadatSylvie Nonin-Lecomte

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